DCDHF Fluorophores for Single-Molecule Imaging in Cells Export

@article{citeulike:3682455, abstract = {There is a persistent need for small-molecule fluorescent labels optimized for single-molecule imaging in the cellular environment. Application of these labels comes with a set of strict requirements: strong absorption, efficient and stable emission, water solubility and membrane permeability, low background emission, and red-shifted absorption to avoid cell autofluorescence. We have designed and characterized several fluorophores, termed ldquoDCDHFrdquo fluorophores, for use in live-cell imaging based on the push-pull design: an amine donor group and a 2-dicyanomethylene-3-cyano-2,5-dihydrofuran (DCDHF) acceptor group, separated by a pi-rich conjugated network. In general, the DCDHF fluorophores are comparatively photostable, sensitive to local environment, and their chemistries and photophysics are tunable to optimize absorption wavelength, membrane affinity, and solubility. Especially valuable are fluorophores with sophisticated photophysics for applications requiring additional facets of control, such as photoactivation. For example, we have reengineered a red-emitting DCDHF fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. This molecule and its relatives provide a new class of bright photoactivatable small-molecule fluorophores, which are needed for super-resolution imaging schemes that require active control (here turning-on) of single-molecule emission.}, address = {Department of Chemistry, Stanford University, Stanford, California 94305 (USA), Fax: (+1) 650-725-0259; Department of Chemistry, Kent State University, Kent, Ohio 44240 (USA)}, author = {Lord, Samuel J. and Conley, Nicholas R. and Lee, Hsiao-Lu D. and Nishimura, Stefanie Y. and Pomerantz, Andrea K. and Willets, Katherine A. and Lu, Zhikuan and Wang, Hui and Liu, Na and Samuel, Reichel and Weber, Ryan and Semyonov, Alexander and He, Meng and Twieg, Robert J. and Moerner, W. E.}, citeulike-article-id = {3682455}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/cphc.200800581}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/121532593/ABSTRACT}, doi = {10.1002/cphc.200800581}, journal = {ChemPhysChem}, keywords = {dcdhf, fluorophores, review, sms}, month = {January}, number = {1}, pages = {55--65}, posted-at = {2008-11-25 02:27:32}, priority = {2}, title = {DCDHF Fluorophores for Single-Molecule Imaging in Cells}, url = {http://dx.doi.org/10.1002/cphc.200800581}, volume = {10}, year = {2009} }

TY - JOUR ID - citeulike:3682455 L3 - citeulike-article-id:3682455 N2 - There is a persistent need for small-molecule fluorescent labels optimized for single-molecule imaging in the cellular environment. Application of these labels comes with a set of strict requirements: strong absorption, efficient and stable emission, water solubility and membrane permeability, low background emission, and red-shifted absorption to avoid cell autofluorescence. We have designed and characterized several fluorophores, termed ldquoDCDHFrdquo fluorophores, for use in live-cell imaging based on the push-pull design: an amine donor group and a 2-dicyanomethylene-3-cyano-2,5-dihydrofuran (DCDHF) acceptor group, separated by a pi-rich conjugated network. In general, the DCDHF fluorophores are comparatively photostable, sensitive to local environment, and their chemistries and photophysics are tunable to optimize absorption wavelength, membrane affinity, and solubility. Especially valuable are fluorophores with sophisticated photophysics for applications requiring additional facets of control, such as photoactivation. For example, we have reengineered a red-emitting DCDHF fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. This molecule and its relatives provide a new class of bright photoactivatable small-molecule fluorophores, which are needed for super-resolution imaging schemes that require active control (here turning-on) of single-molecule emission. IS - 1 JF - ChemPhysChem AD - Department of Chemistry, Stanford University, Stanford, California 94305 (USA), Fax: (+1) 650-725-0259; Department of Chemistry, Kent State University, Kent, Ohio 44240 (USA) EP - 65 TI - DCDHF Fluorophores for Single-Molecule Imaging in Cells VL - 10 SP - 55 KW - dcdhf KW - fluorophores KW - review KW - sms AU - Lord, Samuel AU - Conley, Nicholas AU - Lee, Hsiao-Lu AU - Nishimura, Stefanie AU - Pomerantz, Andrea AU - Willets, Katherine AU - Lu, Zhikuan AU - Wang, Hui AU - Liu, Na AU - Samuel, Reichel AU - Weber, Ryan AU - Semyonov, Alexander AU - He, Meng AU - Twieg, Robert AU - Moerner, WE PY - 2009/01// UR - http://dx.doi.org/10.1002/cphc.200800581 ER -