A Genetically Encoded Bioluminescent Indicator for the Sodium Channel Activity in Living Cells Export

@article{citeulike:4172341, abstract = {doi: 10.1021/ja808669y We have developed a genetically encoded bioluminescent indicator for the Na+ channel, in which Na+-sensitive Gaussia luciferase is fused with the voltage-gated Na+ channel. This indicator is capable of detecting Na+ flow through the pores of Na+ channels. Because of high sensitivity and low background in luminescence assays, the absence of toxicity, and a wide linear dynamic range, this luciferase can be used to generate a novel, genetically encoded Na+ channel indicator. It may provide a high-throughput screening system for drug discovery against Na+ channels, which should be useful in controlling lethal cardiac arrhythmias, epileptic seizures, and intolerable pain associated with terminal stages of cancer. It may also offer a system for monitoring the Na+ channel activity in living cells, which may be useful in illuminating neuronal activity in vivo.}, author = {Kaihara, Asami and Sunami, Akihiko and Kurokawa, Junko and Furukawa, Tetsushi}, citeulike-article-id = {4172341}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ja808669y}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ja808669y}, doi = {10.1021/ja808669y}, journal = {Journal of the American Chemical Society}, keywords = {bioluminescence, biophysics, membrane}, number = {0}, posted-at = {2009-03-13 18:23:39}, priority = {2}, title = {A Genetically Encoded Bioluminescent Indicator for the Sodium Channel Activity in Living Cells}, url = {http://dx.doi.org/10.1021/ja808669y}, volume = {0}, year = {0000} }

TY - JOUR ID - citeulike:4172341 L3 - citeulike-article-id:4172341 N2 - doi: 10.1021/ja808669y We have developed a genetically encoded bioluminescent indicator for the Na+ channel, in which Na+-sensitive Gaussia luciferase is fused with the voltage-gated Na+ channel. This indicator is capable of detecting Na+ flow through the pores of Na+ channels. Because of high sensitivity and low background in luminescence assays, the absence of toxicity, and a wide linear dynamic range, this luciferase can be used to generate a novel, genetically encoded Na+ channel indicator. It may provide a high-throughput screening system for drug discovery against Na+ channels, which should be useful in controlling lethal cardiac arrhythmias, epileptic seizures, and intolerable pain associated with terminal stages of cancer. It may also offer a system for monitoring the Na+ channel activity in living cells, which may be useful in illuminating neuronal activity in vivo. IS - 0 JF - Journal of the American Chemical Society TI - A Genetically Encoded Bioluminescent Indicator for the Sodium Channel Activity in Living Cells VL - 0 KW - bioluminescence KW - biophysics KW - membrane AU - Kaihara, Asami AU - Sunami, Akihiko AU - Kurokawa, Junko AU - Furukawa, Tetsushi PY - 2000/// UR - http://dx.doi.org/10.1021/ja808669y ER -