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Parallax: High Accuracy Three-Dimensional Single Molecule Tracking Using Split Images Export

Nano Letters, Vol. 0, No. 0. (0000)

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Three-dimensional (3D) tracking can provide valuable biological insights that are missing in conventional microscopy. Here we developed a single molecule 3D tracking microscopy technique, named Parallax, with high localization precision and temporal resolution. We demonstrated its capabilities by studying the 3D trafficking of glucose-transporter-4 containing vesicles in living adipocytes as well as the walking path of single myosin VI molecules along actin filaments.


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