Probing Dynein and Kinesin Stepping with Mechanical Manipulation in a Living Cell Export

@article{citeulike:4792067, abstract = {We report a label-free assay for simultaneous optical manipulation and tracking of endogenous lipid droplets as actively transported cargoes in a living mammalian cell with sub-millisecond time resolution. Using an EM-CCD camera as a highly sensitive quadrant detector, we can detect steps of dynein- and kinesin-driven cargoes under known force loads. We can distinguish single and multiple motor-driven cargoes and show that the stall forces for inward and outward transported cargoes are similar. By combining the stall force observable with the ability to detect individual steps, we can characterize kinesin- and dynein-driven active transport in different force regimes.}, address = {Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138 (USA), Fax: (+1) 617 496 8709}, author = {Peter a and Xie, Sunney X.}, citeulike-article-id = {4792067}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/cphc.200900113}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/122440825/ABSTRACT}, doi = {10.1002/cphc.200900113}, journal = {ChemPhysChem}, keywords = {biophysics, motors, sms, trapping}, number = {9999}, pages = {NA+}, posted-at = {2009-06-10 01:21:21}, priority = {2}, title = {Probing Dynein and Kinesin Stepping with Mechanical Manipulation in a Living Cell}, url = {http://dx.doi.org/10.1002/cphc.200900113}, volume = {9999}, year = {2009} }

TY - JOUR ID - citeulike:4792067 L3 - citeulike-article-id:4792067 N2 - We report a label-free assay for simultaneous optical manipulation and tracking of endogenous lipid droplets as actively transported cargoes in a living mammalian cell with sub-millisecond time resolution. Using an EM-CCD camera as a highly sensitive quadrant detector, we can detect steps of dynein- and kinesin-driven cargoes under known force loads. We can distinguish single and multiple motor-driven cargoes and show that the stall forces for inward and outward transported cargoes are similar. By combining the stall force observable with the ability to detect individual steps, we can characterize kinesin- and dynein-driven active transport in different force regimes. IS - 9999 JF - ChemPhysChem AD - Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138 (USA), Fax: (+1) 617 496 8709 TI - Probing Dynein and Kinesin Stepping with Mechanical Manipulation in a Living Cell VL - 9999 SP - NA KW - biophysics KW - motors KW - sms KW - trapping AU - Peter a AU - Xie, Sunney PY - 2009/// UR - http://dx.doi.org/10.1002/cphc.200900113 ER -