Nanoscale separation of molecular species based on their rotational mobility Export

@article{citeulike:4863103, abstract = {We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells.}, author = {Testa, Ilaria and Sch\"{o}nle, Andreas and Middendorff and Geisler, Claudia and Medda, Rebecca and Wurm, Christian A. and Stiel, Andre C. and Jakobs, Stefan and Bossi, Mariano and Eggeling, Christian and Hell, Stefan W. and Egner, Alexander}, citeulike-article-id = {4863103}, citeulike-linkout-0 = {http://dx.doi.org/10.1364/OE.16.021093}, citeulike-linkout-1 = {http://www.opticsinfobase.org/abstract.cfm?id=175203}, day = {8}, doi = {10.1364/OE.16.021093}, journal = {Opt. Express}, keywords = {data-analysis, microscopy, sms, super-resolution}, month = {December}, number = {25}, pages = {21093--21104}, posted-at = {2009-06-19 05:13:12}, priority = {2}, publisher = {OSA}, title = {Nanoscale separation of molecular species based on their rotational mobility}, url = {http://dx.doi.org/10.1364/OE.16.021093}, volume = {16}, year = {2008} }

TY - JOUR ID - citeulike:4863103 L3 - citeulike-article-id:4863103 N2 - We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells. IS - 25 JF - Opt. Express EP - 21104 TI - Nanoscale separation of molecular species based on their rotational mobility PB - OSA VL - 16 SP - 21093 KW - data-analysis KW - microscopy KW - sms KW - super-resolution AU - Testa, Ilaria AU - Schönle, Andreas AU - Middendorff AU - Geisler, Claudia AU - Medda, Rebecca AU - Wurm, Christian AU - Stiel, Andre AU - Jakobs, Stefan AU - Bossi, Mariano AU - Eggeling, Christian AU - Hell, Stefan AU - Egner, Alexander PY - 2008/12/08/ UR - http://dx.doi.org/10.1364/OE.16.021093 ER -