Advances in light microscopy for neuroscience. Export

@article{citeulike:4967166, abstract = {Since the work of Golgi and Cajal, light microscopy has remained a key tool for neuroscientists to observe cellular properties. Ongoing advances have enabled new experimental capabilities using light to inspect the nervous system across multiple spatial scales, including ultrastructural scales finer than the optical diffraction limit. Other progress permits functional imaging at faster speeds, at greater depths in brain tissue, and over larger tissue volumes than previously possible. Portable, miniaturized fluorescence microscopes now allow brain imaging in freely behaving mice. Complementary progress on animal preparations has enabled imaging in head-restrained behaving animals, as well as time-lapse microscopy studies in the brains of live subjects. Mouse genetic approaches permit mosaic and inducible fluorescence-labeling strategies, whereas intrinsic contrast mechanisms allow in vivo imaging of animals and humans without use of exogenous markers. This review surveys such advances and highlights emerging capabilities of particular interest to neuroscientists.}, author = {Wilt, Brian A. and Burns, Laurie D. and Wei Ho, Eric Tatt T. and Ghosh, Kunal K. and Mukamel, Eran A. and Schnitzer, Mark J.}, citeulike-article-id = {4967166}, citeulike-linkout-0 = {http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.neuro.051508.135540}, citeulike-linkout-1 = {http://dx.doi.org/10.1146/annurev.neuro.051508.135540}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19555292}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19555292}, doi = {10.1146/annurev.neuro.051508.135540}, issn = {1545-4126}, journal = {Annual review of neuroscience}, keywords = {microscopy, review, super-resolution}, number = {1}, pages = {435--506}, posted-at = {2009-06-26 17:05:35}, priority = {2}, title = {Advances in light microscopy for neuroscience.}, url = {http://dx.doi.org/10.1146/annurev.neuro.051508.135540}, volume = {32}, year = {2009} }

TY - JOUR ID - citeulike:4967166 L3 - citeulike-article-id:4967166 N2 - Since the work of Golgi and Cajal, light microscopy has remained a key tool for neuroscientists to observe cellular properties. Ongoing advances have enabled new experimental capabilities using light to inspect the nervous system across multiple spatial scales, including ultrastructural scales finer than the optical diffraction limit. Other progress permits functional imaging at faster speeds, at greater depths in brain tissue, and over larger tissue volumes than previously possible. Portable, miniaturized fluorescence microscopes now allow brain imaging in freely behaving mice. Complementary progress on animal preparations has enabled imaging in head-restrained behaving animals, as well as time-lapse microscopy studies in the brains of live subjects. Mouse genetic approaches permit mosaic and inducible fluorescence-labeling strategies, whereas intrinsic contrast mechanisms allow in vivo imaging of animals and humans without use of exogenous markers. This review surveys such advances and highlights emerging capabilities of particular interest to neuroscientists. IS - 1 JF - Annual review of neuroscience SN - 1545-4126 EP - 506 TI - Advances in light microscopy for neuroscience. VL - 32 SP - 435 KW - microscopy KW - review KW - super-resolution AU - Wilt, Brian AU - Burns, Laurie AU - Wei Ho, Eric Tatt AU - Ghosh, Kunal AU - Mukamel, Eran AU - Schnitzer, Mark PY - 2009/// UR - http://dx.doi.org/10.1146/annurev.neuro.051508.135540 ER -