Limits on Fluorescence Detected Circular Dichroism of Single Helicene Molecules Export

@article{citeulike:5016338, abstract = {Fluorescent imaging of single helicene molecules is applied to study the optical activity of chiral fluorophores. In contrast to the previous report by Hassey et al. (Science 2006, 314, 1437), the dissymmetry factors of single chiral fluorophores are found not to differ significantly from the bulk value of |g| < 10−4 at 457 nm. Linear dichroism and birefringence of the dichroic mirror inside the fluorescence microscope change the polarization state of the incoming laser beam significantly; i.e., circular polarized light sent into the microscope becomes highly elliptically polarized after reflection from the dichroic mirror. Compensation for this effect should be made to avoid artifacts brought by linear dichroism in single immobilized molecules.}, author = {Tang, Yiqiao and Cook, Timothy A. and Cohen, Adam E.}, citeulike-article-id = {5016338}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/jp903598t}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/jp903598t}, day = {4}, doi = {10.1021/jp903598t}, journal = {The Journal of Physical Chemistry A}, keywords = {microscopy, optics, sms}, month = {June}, number = {22}, pages = {6213--6216}, posted-at = {2009-06-29 23:56:57}, priority = {2}, title = {Limits on Fluorescence Detected Circular Dichroism of Single Helicene Molecules}, url = {http://dx.doi.org/10.1021/jp903598t}, volume = {113}, year = {2009} }

TY - JOUR ID - citeulike:5016338 L3 - citeulike-article-id:5016338 N2 - Fluorescent imaging of single helicene molecules is applied to study the optical activity of chiral fluorophores. In contrast to the previous report by Hassey et al. (Science 2006, 314, 1437), the dissymmetry factors of single chiral fluorophores are found not to differ significantly from the bulk value of |g| < 10−4 at 457 nm. Linear dichroism and birefringence of the dichroic mirror inside the fluorescence microscope change the polarization state of the incoming laser beam significantly; i.e., circular polarized light sent into the microscope becomes highly elliptically polarized after reflection from the dichroic mirror. Compensation for this effect should be made to avoid artifacts brought by linear dichroism in single immobilized molecules. IS - 22 JF - The Journal of Physical Chemistry A EP - 6216 TI - Limits on Fluorescence Detected Circular Dichroism of Single Helicene Molecules VL - 113 SP - 6213 KW - microscopy KW - optics KW - sms AU - Tang, Yiqiao AU - Cook, Timothy AU - Cohen, Adam PY - 2009/06/04/ UR - http://dx.doi.org/10.1021/jp903598t ER -