Fluorescein Redirects a Ruthenium−Octaarginine Conjugate to the Nucleus Export

@article{citeulike:5017232, abstract = {The cellular uptake and localization of a Ru−octaarginine conjugate with and without an appended fluorescein are compared. The inherent luminescence of the Ru(II) dipyridophenazine complex allows observation of its uptake without the addition of a fluorophore. Ru−octaarginine−fluorescein stains the cytosol, nuclei, and nucleoli of HeLa cells under conditions where the Ru−octaarginine conjugate without fluorescein shows only punctate cytoplasmic labeling. At higher concentrations, however, Ru−octaarginine without the fluorescein tag does exhibit cytoplasmic, nuclear, and nucleolar staining. Attaching fluorescein to Ru−octaarginine lowers the threshold concentration required for diffuse cytoplasmic labeling and nuclear entry. Hence, the localization of the fluorophore-bound peptide cannot serve as a proxy for that of the free peptide.}, author = {Puckett, Cindy A. and Barton, Jacqueline K.}, citeulike-article-id = {5017232}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ja9025165}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ja9025165}, day = {1}, doi = {10.1021/ja9025165}, journal = {Journal of the American Chemical Society}, keywords = {bioconjugation, biophysics}, month = {July}, number = {25}, pages = {8738--8739}, posted-at = {2009-06-30 02:04:50}, priority = {2}, title = {Fluorescein Redirects a Ruthenium−Octaarginine Conjugate to the Nucleus}, url = {http://dx.doi.org/10.1021/ja9025165}, volume = {131}, year = {2009} }

TY - JOUR ID - citeulike:5017232 L3 - citeulike-article-id:5017232 N2 - The cellular uptake and localization of a Ru−octaarginine conjugate with and without an appended fluorescein are compared. The inherent luminescence of the Ru(II) dipyridophenazine complex allows observation of its uptake without the addition of a fluorophore. Ru−octaarginine−fluorescein stains the cytosol, nuclei, and nucleoli of HeLa cells under conditions where the Ru−octaarginine conjugate without fluorescein shows only punctate cytoplasmic labeling. At higher concentrations, however, Ru−octaarginine without the fluorescein tag does exhibit cytoplasmic, nuclear, and nucleolar staining. Attaching fluorescein to Ru−octaarginine lowers the threshold concentration required for diffuse cytoplasmic labeling and nuclear entry. Hence, the localization of the fluorophore-bound peptide cannot serve as a proxy for that of the free peptide. IS - 25 JF - Journal of the American Chemical Society EP - 8739 TI - Fluorescein Redirects a Ruthenium−Octaarginine Conjugate to the Nucleus VL - 131 SP - 8738 KW - bioconjugation KW - biophysics AU - Puckett, Cindy AU - Barton, Jacqueline PY - 2009/07/01/ UR - http://dx.doi.org/10.1021/ja9025165 ER -