Fluorescent Lifetime Trajectories of a Single Fluorophore Reveal Reaction Intermediates During Transcription Initiation Export

@article{citeulike:5201114, abstract = {PMID: 19552410 Single molecule (SM) techniques are relatively new additions to the field of biophysics that allow one to manipulate individual molecules and study their behavior. To make these studies more relevant to what actually happens in the cell, one needs to move beyond the studies of individual molecules in isolation and study many different molecules working in concert. This presents a technical challenge as most SM experiments measure only one observable as a function of time, whereas complex biomolecular systems require multidimensional SM analysis. Förster resonance energy transfer (FRET) is one of the most common single molecule approaches and can report on the real time distance changes. However, FRET requires two fluorophores which will ultimately limit the degree of multiplexing in future SM applications. It will be useful if a single fluorophore can be used to provide equivalent information. In this communication, we show that fluorescence lifetime analysis of a single Cy3 fluorophore attached to the promoter region of the DNA can be used to reveal transient reaction intermediates during transcription initiation by T7 RNA polymerase. This work represents the first demonstration of real-time biochemical reactions observed via single molecule fluorescence lifetime trajectories of immobilized molecules.}, author = {Sorokina, Maria and Koh, Hye-Ran and Patel, Smita S. and Ha, Taekjip}, citeulike-article-id = {5201114}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ja902861f}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ja902861f}, day = {22}, doi = {10.1021/ja902861f}, journal = {Journal of the American Chemical Society}, keywords = {biophysics, enzymes, sms}, month = {July}, number = {28}, pages = {9630--9631}, posted-at = {2009-11-04 19:06:45}, priority = {2}, title = {Fluorescent Lifetime Trajectories of a Single Fluorophore Reveal Reaction Intermediates During Transcription Initiation}, url = {http://dx.doi.org/10.1021/ja902861f}, volume = {131}, year = {2009} }

TY - JOUR ID - citeulike:5201114 L3 - citeulike-article-id:5201114 N2 - PMID: 19552410 Single molecule (SM) techniques are relatively new additions to the field of biophysics that allow one to manipulate individual molecules and study their behavior. To make these studies more relevant to what actually happens in the cell, one needs to move beyond the studies of individual molecules in isolation and study many different molecules working in concert. This presents a technical challenge as most SM experiments measure only one observable as a function of time, whereas complex biomolecular systems require multidimensional SM analysis. Förster resonance energy transfer (FRET) is one of the most common single molecule approaches and can report on the real time distance changes. However, FRET requires two fluorophores which will ultimately limit the degree of multiplexing in future SM applications. It will be useful if a single fluorophore can be used to provide equivalent information. In this communication, we show that fluorescence lifetime analysis of a single Cy3 fluorophore attached to the promoter region of the DNA can be used to reveal transient reaction intermediates during transcription initiation by T7 RNA polymerase. This work represents the first demonstration of real-time biochemical reactions observed via single molecule fluorescence lifetime trajectories of immobilized molecules. IS - 28 JF - Journal of the American Chemical Society EP - 9631 TI - Fluorescent Lifetime Trajectories of a Single Fluorophore Reveal Reaction Intermediates During Transcription Initiation VL - 131 SP - 9630 KW - biophysics KW - enzymes KW - sms AU - Sorokina, Maria AU - Koh, Hye-Ran AU - Patel, Smita AU - Ha, Taekjip PY - 2009/07/22/ UR - http://dx.doi.org/10.1021/ja902861f ER -