Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability Export

@article{citeulike:5743632, abstract = {PMID: 19772358 A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface−sample contacts and allows the in situ addition and combination of other reagents.}, author = {Lemke, Edward A. and Gambin, Yann and Vandelinder, Virginia and Brustad, Eric M. and Liu, Hsiao-Wei and Schultz, Peter G. and Groisman, Alex and Deniz, Ashok A.}, citeulike-article-id = {5743632}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ja9027023}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ja9027023}, day = {30}, doi = {10.1021/ja9027023}, journal = {Journal of the American Chemical Society}, keywords = {microfluidics, photobleaching}, month = {September}, number = {38}, pages = {13610--13612}, posted-at = {2009-09-08 18:50:40}, priority = {2}, title = {Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability}, url = {http://dx.doi.org/10.1021/ja9027023}, volume = {131}, year = {2009} }

TY - JOUR ID - citeulike:5743632 L3 - citeulike-article-id:5743632 N2 - PMID: 19772358 A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface−sample contacts and allows the in situ addition and combination of other reagents. IS - 38 JF - Journal of the American Chemical Society EP - 13612 TI - Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability VL - 131 SP - 13610 KW - microfluidics KW - photobleaching AU - Lemke, Edward AU - Gambin, Yann AU - Vandelinder, Virginia AU - Brustad, Eric AU - Liu, Hsiao-Wei AU - Schultz, Peter AU - Groisman, Alex AU - Deniz, Ashok PY - 2009/09/30/ UR - http://dx.doi.org/10.1021/ja9027023 ER -