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Super-Resolution Imaging with Small Organic Fluorophores Export

by: Mike Heilemann, Sebastian van de Linde, Anindita Mukherjee, Markus Sauer
Angewandte Chemie International Edition, Vol. 48, No. 37. (2009), pp. 6903-6908.

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blinking fluorophores photoswitchable super-resolution

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What's your color? A universal and facile method for reversible photoswitching of commercially available Alexa Fluor and ATTO dyes spanning the entire visible range yields an optical resolution of approximately 20 nm. The intriguing simplicity of the method facilitates applications and opens avenues for multicolor super-resolution imaging in fixed and living cells.


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