Overhauser enhanced magnetic resonance imaging for tumor oximetry: Coregistration of tumor anatomy and tissue oxygen concentration Export

@article{citeulike:1247221, abstract = {An efficient noninvasive method for in vivo imaging of tumor oxygenation by using a low-field magnetic resonance scanner and a paramagnetic contrast agent is described. The methodology is based on Overhauser enhanced magnetic resonance imaging (OMRI), a functional imaging technique. OMRI experiments were performed on tumor-bearing mice (squamous cell carcinoma) by i.v. administration of the contrast agent Oxo63 (a highly derivatized triarylmethyl radical) at nontoxic doses in the range of 2-7 mmol/kg either as a bolus or as a continuous infusion. Spatially resolved pO2 (oxygen concentration) images from OMRI experiments of tumor-bearing mice exhibited heterogeneous oxygenation profiles and revealed regions of hypoxia in tumors (<10 mmHg; 1 mmHg = 133 Pa). Oxygenation of tumors was enhanced on carbogen (95\% O2/5\% CO2) inhalation. The pO2 measurements from OMRI were found to be in agreement with those obtained by independent polarographic measurements using a pO2 Eppendorf electrode. This work illustrates that anatomically coregistered pO2 maps of tumors can be readily obtained by combining the good anatomical resolution of water proton-based MRI, and the superior pO2 sensitivity of EPR. OMRI affords the opportunity to perform noninvasive and repeated pO2 measurements of the same animal with useful spatial ([approx]1 mm) and temporal (2 min) resolution, making this method a powerful imaging modality for small animal research to understand tumor physiology and potentially for human applications. 10.1073/pnas.042671399}, author = {Krishna, Murali C. and English, Sean and Yamada, Kenichi and Yoo, John and Murugesan, Ramachandran and Devasahayam, Nallathamby and Cook, John A. and Golman, Klaes and Ardenkjaer-Larsen, Jan H. and Subramanian, Sankaran and Mitchell, James B.}, citeulike-article-id = {1247221}, citeulike-linkout-0 = {http://www.pnas.org/cgi/content/abstract/99/4/2216}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11854518}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11854518}, day = {19}, journal = {PNAS}, keywords = {epr, oxo63, oxygen\_measurement}, month = {February}, number = {4}, pages = {2216--2221}, posted-at = {2007-04-24 09:08:03}, priority = {2}, title = {Overhauser enhanced magnetic resonance imaging for tumor oximetry: Coregistration of tumor anatomy and tissue oxygen concentration}, url = {http://www.pnas.org/cgi/content/abstract/99/4/2216}, volume = {99}, year = {2002} }

TY - JOUR ID - citeulike:1247221 L3 - citeulike-article-id:1247221 N2 - An efficient noninvasive method for in vivo imaging of tumor oxygenation by using a low-field magnetic resonance scanner and a paramagnetic contrast agent is described. The methodology is based on Overhauser enhanced magnetic resonance imaging (OMRI), a functional imaging technique. OMRI experiments were performed on tumor-bearing mice (squamous cell carcinoma) by i.v. administration of the contrast agent Oxo63 (a highly derivatized triarylmethyl radical) at nontoxic doses in the range of 2-7 mmol/kg either as a bolus or as a continuous infusion. Spatially resolved pO2 (oxygen concentration) images from OMRI experiments of tumor-bearing mice exhibited heterogeneous oxygenation profiles and revealed regions of hypoxia in tumors (<10 mmHg; 1 mmHg = 133 Pa). Oxygenation of tumors was enhanced on carbogen (95% O2/5% CO2) inhalation. The pO2 measurements from OMRI were found to be in agreement with those obtained by independent polarographic measurements using a pO2 Eppendorf electrode. This work illustrates that anatomically coregistered pO2 maps of tumors can be readily obtained by combining the good anatomical resolution of water proton-based MRI, and the superior pO2 sensitivity of EPR. OMRI affords the opportunity to perform noninvasive and repeated pO2 measurements of the same animal with useful spatial ([approx]1 mm) and temporal (2 min) resolution, making this method a powerful imaging modality for small animal research to understand tumor physiology and potentially for human applications. 10.1073/pnas.042671399 IS - 4 JF - PNAS EP - 2221 TI - Overhauser enhanced magnetic resonance imaging for tumor oximetry: Coregistration of tumor anatomy and tissue oxygen concentration VL - 99 SP - 2216 KW - epr KW - oxo63 KW - oxygen_measurement AU - Krishna, Murali AU - English, Sean AU - Yamada, Kenichi AU - Yoo, John AU - Murugesan, Ramachandran AU - Devasahayam, Nallathamby AU - Cook, John AU - Golman, Klaes AU - Ardenkjaer-Larsen, Jan AU - Subramanian, Sankaran AU - Mitchell, James PY - 2002/02/19/ UR - http://www.pnas.org/cgi/content/abstract/99/4/2216 ER -