MCP-1 Expression in CNS-1 Astrocytoma Cells: Implications for Macrophage Infiltration into Tumors In vivo Export

@article{citeulike:1512906, abstract = {Gliomas are among the most resistant tumors to conventional anti-tumor therapy, and are typified by their highly infiltrative nature and ill-defined borders. Macrophages constitute a major proportion of the tumor cell mass in both primary human gliomas and as shown here, a CNS-1 glioma model. The objective of this study was to identify tumor-cell-derived chemotactic factor(s) which participate in macrophage recruitment into tumors in vivo. This study demonstrates the constitutive expression of monocyte chemoattractant protein-1 (MCP-1), a potent monocyte chemoattractant, by the rat astrocytoma cell line CNS-1. Characterization of cytokine expression by CNS-1 cells in vitro revealed the constitutive expression of TGF-{\ss} but not other proinflammatory cytokines. However, numerous cytokines were detected in CNS-1 tumors in vivo including Lt{\ss}, IL-1a, IL-1{\ss}, TNF-a, TNF-{\ss}, IL-10, and IFN-?. Attenuation of MCP-1 release from CNS-1 cells using an anti-sense approach revealed no significant alterations in macrophage infiltration into tumors in vivo, suggesting redundancy in the signal(s) involved in macrophage recruitment. Depletion of peripheral macrophages using liposome-encapsulated clodronate revealed no significant differences in tumor growth or in the degree of macrophage infiltration into CNS-1 tumors in vivo. These results indicate that CNS-1 cells produce chemotactic factors which likely participate in macrophage recruitment into tumors in vivo. Whether or not macrophage recruitment confers a growth advantage for the tumor remains to be determined.}, author = {Kielian, Tammy and van Rooijen, Nico and Hickey, William F.}, citeulike-article-id = {1512906}, citeulike-linkout-0 = {http://dx.doi.org/10.1023/A:1014495613455}, day = {1}, doi = {10.1023/A:1014495613455}, journal = {Journal of Neuro-Oncology}, keywords = {brain, cancer, glioma, invasion, macrophage}, month = {January}, number = {1}, pages = {1--12}, posted-at = {2007-07-30 15:03:14}, priority = {2}, title = {MCP-1 Expression in CNS-1 Astrocytoma Cells: Implications for Macrophage Infiltration into Tumors In vivo}, url = {http://dx.doi.org/10.1023/A:1014495613455}, volume = {56}, year = {2002} }

TY - JOUR ID - citeulike:1512906 L3 - citeulike-article-id:1512906 N2 - Gliomas are among the most resistant tumors to conventional anti-tumor therapy, and are typified by their highly infiltrative nature and ill-defined borders. Macrophages constitute a major proportion of the tumor cell mass in both primary human gliomas and as shown here, a CNS-1 glioma model. The objective of this study was to identify tumor-cell-derived chemotactic factor(s) which participate in macrophage recruitment into tumors in vivo. This study demonstrates the constitutive expression of monocyte chemoattractant protein-1 (MCP-1), a potent monocyte chemoattractant, by the rat astrocytoma cell line CNS-1. Characterization of cytokine expression by CNS-1 cells in vitro revealed the constitutive expression of TGF-ß but not other proinflammatory cytokines. However, numerous cytokines were detected in CNS-1 tumors in vivo including Ltß, IL-1a, IL-1ß, TNF-a, TNF-ß, IL-10, and IFN-?. Attenuation of MCP-1 release from CNS-1 cells using an anti-sense approach revealed no significant alterations in macrophage infiltration into tumors in vivo, suggesting redundancy in the signal(s) involved in macrophage recruitment. Depletion of peripheral macrophages using liposome-encapsulated clodronate revealed no significant differences in tumor growth or in the degree of macrophage infiltration into CNS-1 tumors in vivo. These results indicate that CNS-1 cells produce chemotactic factors which likely participate in macrophage recruitment into tumors in vivo. Whether or not macrophage recruitment confers a growth advantage for the tumor remains to be determined. IS - 1 JF - Journal of Neuro-Oncology EP - 12 TI - MCP-1 Expression in CNS-1 Astrocytoma Cells: Implications for Macrophage Infiltration into Tumors In vivo VL - 56 SP - 1 KW - brain KW - cancer KW - glioma KW - invasion KW - macrophage AU - Kielian, Tammy AU - van Rooijen, Nico AU - Hickey, William PY - 2002/01/01/ UR - http://dx.doi.org/10.1023/A:1014495613455 ER -