Apoptosis of macrophages induced by liposome-mediated intracellular delivery of clodronate and propamidine Export

@article{citeulike:1524359, abstract = {Liposomes can be used as vehicles for intracellular delivery of drugs into phagocytic cells. Clodronate and propamidine, delivered into macrophages in this way, will kill these cells as a result of intracellular accumulation and irreversible metabolic damage. The so-called liposome-mediated macrophage `suicide' approach, which is based on this principle, is now frequently applied in studies aimed at unravelling macrophage function. In the present study, the mechanism of phagocytic cell death induced by liposome encapsulated drugs was investigated `in vitro'. Peritoneal macrophages and macrophages of the RAW 264 cell line were cultured in the presence of the liposome encapsulated drugs clodronate, propamidine and several forms of ethylenediaminetetraacetic acid (EDTA). The results obtained suggest that apoptotic death is induced in phagocytic cells both by liposomally delivered clodronate and by liposomally delivered propamidine. Although intracellular EDTA did induce apoptosis in a minority of the experiments, the results support earlier findings that EDTA does not deplete macrophages as effectively as clodronate and propamidine.}, author = {van Rooijen, Nico and Sanders, Annemarie and van den Berg, Timo K.}, citeulike-article-id = {1524359}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0022-1759(96)00056-7}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6T2Y-3VYTXR1-B/2/52506c3737c698c6fa3f7551ce7d3b42}, day = {14}, doi = {10.1016/0022-1759(96)00056-7}, journal = {Journal of Immunological Methods}, keywords = {apoptosis, clodronate, liposomes, macrophage}, month = {June}, number = {1}, pages = {93--99}, posted-at = {2007-07-31 09:48:13}, priority = {2}, title = {Apoptosis of macrophages induced by liposome-mediated intracellular delivery of clodronate and propamidine}, url = {http://dx.doi.org/10.1016/0022-1759(96)00056-7}, volume = {193}, year = {1996} }

TY - JOUR ID - citeulike:1524359 L3 - citeulike-article-id:1524359 N2 - Liposomes can be used as vehicles for intracellular delivery of drugs into phagocytic cells. Clodronate and propamidine, delivered into macrophages in this way, will kill these cells as a result of intracellular accumulation and irreversible metabolic damage. The so-called liposome-mediated macrophage `suicide' approach, which is based on this principle, is now frequently applied in studies aimed at unravelling macrophage function. In the present study, the mechanism of phagocytic cell death induced by liposome encapsulated drugs was investigated `in vitro'. Peritoneal macrophages and macrophages of the RAW 264 cell line were cultured in the presence of the liposome encapsulated drugs clodronate, propamidine and several forms of ethylenediaminetetraacetic acid (EDTA). The results obtained suggest that apoptotic death is induced in phagocytic cells both by liposomally delivered clodronate and by liposomally delivered propamidine. Although intracellular EDTA did induce apoptosis in a minority of the experiments, the results support earlier findings that EDTA does not deplete macrophages as effectively as clodronate and propamidine. IS - 1 JF - Journal of Immunological Methods EP - 99 TI - Apoptosis of macrophages induced by liposome-mediated intracellular delivery of clodronate and propamidine VL - 193 SP - 93 KW - apoptosis KW - clodronate KW - liposomes KW - macrophage AU - van Rooijen, Nico AU - Sanders, Annemarie AU - van den Berg, Timo PY - 1996/06/14/ UR - http://dx.doi.org/10.1016/0022-1759(96)00056-7 ER -