Cerebral lactate turnover after electroshock: In vivo measurements by 1H/13C magnetic resonance spectroscopy Export

@article{citeulike:2757874, abstract = {We reported earlier that brain activation by 10 s of cortical electroshock caused prolonged elevation of brain lactate without significant change in intracellular pH, brain high-energy phosphorylated metabolites, or blood gases. The metabolic state of the elevated lactate has been investigated in further experiments using combined, in vivo 1H-observed 13C-edited nuclear magnetic resonance spectroscopy (NMRS), homonuclear J-edited 1H-NMRS, and high-resolution 1H-NMRS of perchloric acid extracts to monitor concentrations and 13C-isotopic fractions of brain and blood lactate and glucose. We now report that electroshock-elevated lactate pool in rabbit brain approaches equilibrium with blood glucose within 1 h. There was nearly complete turnover of the raised lactate pool in brain; any pool of metabolically inactive lactate could not have been >5\% of the total. In the same experiments, blood lactate underwent <50\% turnover in 1 h. The new 1H- spectroscopic methods used for these experiments are readily adaptable for the study of human brain and may be useful in characterizing the metabolic state of elevated lactate pools associated with epilepsy, stroke, trauma, tumors, and other pathological conditions.}, address = {Department of Neurology, Yale University, 333 Cedar Street, New Haven, CT 06510, United States}, author = {Petroff, O. A. C. and Novotny, E. J. and Avison, M. and Rothman, D. L. and Alger, J. R. and Ogino, T. and Shulman, G. I. and Prichard, J. W.}, citeulike-article-id = {2757874}, citeulike-linkout-0 = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-0026756109}, journal = {Journal of Cerebral Blood Flow and Metabolism}, keywords = {13c, brain, lactate, metabolism, stroke, turnover}, number = {6}, pages = {1022--1029}, posted-at = {2008-05-05 14:20:33}, priority = {2}, title = {Cerebral lactate turnover after electroshock: In vivo measurements by 1H/13C magnetic resonance spectroscopy}, url = {http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-0026756109}, volume = {12}, year = {1992} }

TY - JOUR ID - citeulike:2757874 L3 - citeulike-article-id:2757874 N2 - We reported earlier that brain activation by 10 s of cortical electroshock caused prolonged elevation of brain lactate without significant change in intracellular pH, brain high-energy phosphorylated metabolites, or blood gases. The metabolic state of the elevated lactate has been investigated in further experiments using combined, in vivo 1H-observed 13C-edited nuclear magnetic resonance spectroscopy (NMRS), homonuclear J-edited 1H-NMRS, and high-resolution 1H-NMRS of perchloric acid extracts to monitor concentrations and 13C-isotopic fractions of brain and blood lactate and glucose. We now report that electroshock-elevated lactate pool in rabbit brain approaches equilibrium with blood glucose within 1 h. There was nearly complete turnover of the raised lactate pool in brain; any pool of metabolically inactive lactate could not have been >5% of the total. In the same experiments, blood lactate underwent <50% turnover in 1 h. The new 1H- spectroscopic methods used for these experiments are readily adaptable for the study of human brain and may be useful in characterizing the metabolic state of elevated lactate pools associated with epilepsy, stroke, trauma, tumors, and other pathological conditions. IS - 6 JF - Journal of Cerebral Blood Flow and Metabolism AD - Department of Neurology, Yale University, 333 Cedar Street, New Haven, CT 06510, United States EP - 1029 TI - Cerebral lactate turnover after electroshock: In vivo measurements by 1H/13C magnetic resonance spectroscopy VL - 12 SP - 1022 KW - 13c KW - brain KW - lactate KW - metabolism KW - stroke KW - turnover AU - Petroff, OAC AU - Novotny, EJ AU - Avison, M AU - Rothman, DL AU - Alger, JR AU - Ogino, T AU - Shulman, GI AU - Prichard, JW PY - 1992/// UR - http://www.scopus.com/record/display.url?view=extended&origin=resultslist&eid=2-s2.0-0026756109 ER -