Detection of apoptosis using the C2A domain of synaptotagmin I. Export

@article{citeulike:710956, abstract = {Binding of annexin V or the C2A domain of synaptotagmin I to phosphatidylserine expressed on the surface of apoptotic cells can, when labeled with appropriate probe molecules, be used to detect the presence of apoptosis using radionuclide, magnetic resonance, and optical imaging techniques. The preparation of a biotinylated C2A-GST fusion protein is described, and its capability, when used in conjunction with fluorescein-labeled streptavidin, of detecting apoptotic cells by flow cytometry is compared directly with the performance of a commercial preparation of fluorescein-labeled annexin V. Biotinylated C2A-GST, when used in conjunction with streptavidin-conjugated superparamagnetic iron oxide nanoparticles or Gd-chelate-avidin conjugates, was shown to be capable of detecting apoptotic cells using T(2)-weighted or T(1)-weighted magnetic resonance imaging experiments, respectively.}, address = {Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK.}, author = {Jung, H. I. and Kettunen, M. I. and Davletov, B. and Brindle, K. M.}, citeulike-article-id = {710956}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/bc049899q}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/bc049899q}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15366950}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15366950}, doi = {10.1021/bc049899q}, issn = {1043-1802}, journal = {Bioconjug Chem}, keywords = {apoptosis, phosphatidylserine}, number = {5}, pages = {983--987}, posted-at = {2006-06-26 12:46:54}, priority = {2}, title = {Detection of apoptosis using the C2A domain of synaptotagmin I.}, url = {http://dx.doi.org/10.1021/bc049899q}, volume = {15}, year = {2004} }

TY - JOUR ID - citeulike:710956 L3 - citeulike-article-id:710956 N2 - Binding of annexin V or the C2A domain of synaptotagmin I to phosphatidylserine expressed on the surface of apoptotic cells can, when labeled with appropriate probe molecules, be used to detect the presence of apoptosis using radionuclide, magnetic resonance, and optical imaging techniques. The preparation of a biotinylated C2A-GST fusion protein is described, and its capability, when used in conjunction with fluorescein-labeled streptavidin, of detecting apoptotic cells by flow cytometry is compared directly with the performance of a commercial preparation of fluorescein-labeled annexin V. Biotinylated C2A-GST, when used in conjunction with streptavidin-conjugated superparamagnetic iron oxide nanoparticles or Gd-chelate-avidin conjugates, was shown to be capable of detecting apoptotic cells using T(2)-weighted or T(1)-weighted magnetic resonance imaging experiments, respectively. IS - 5 JF - Bioconjug Chem SN - 1043-1802 AD - Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, UK. EP - 987 TI - Detection of apoptosis using the C2A domain of synaptotagmin I. VL - 15 SP - 983 KW - apoptosis KW - phosphatidylserine AU - Jung, HI AU - Kettunen, MI AU - Davletov, B AU - Brindle, KM PY - 2004///t UR - http://dx.doi.org/10.1021/bc049899q ER -