Pyruvate transport across the plasma membrane of the bloodstream form of Trypanosoma brucei is mediated by a facilitated diffusion carrier Export

@article{citeulike:764230, abstract = {The characteristics of pyruvate transport across the plasma membrane in the bloodstream form of Trypanosoma brucei were studied using [14C]pyruvate in combination with the silicone-oil centrifugation technique. We present evidence for the existence of a facilitated diffusion carrier in the plasma membrane of T. brucei which specifically mediates the translocation of pyruvate. The uptake of pyruvate followed saturation kinetics (Km 1.96 +/- 0.28 mM; Cmax 36.61 +/- 1.15 nmol pyruvate/30 sec.mg protein), after correction of the data for a nonsaturable diffusion component. The uptake of pyruvate was competitively inhibited by a number of (oxo)monocarboxylic acids, including pyruvate analogs and metabolically related substances, but not by L-lactate. The transport exhibited the phenomenon of transacceleration, indicative for the involvement of a facilitated diffusion carrier. The carrier is highly specific for pyruvate and differs from other known monocarboxylate carriers present in the mitochondrial and/or plasma membrane of other eukaryotic cells in that it does not transport L-lactate.}, author = {Wiemer, Erik A. and Ter and Michels, Paul A. and Opperdoes, Fred R.}, citeulike-article-id = {764230}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0006-291X(92)90694-G}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WBK-4F03B28-17K/2/9da0182140fd1e6eaf1a69c323fd32fe}, day = {30}, doi = {10.1016/0006-291X(92)90694-G}, journal = {Biochemical and Biophysical Research Communications}, keywords = {14c, pyruvate-transport}, month = {April}, number = {2}, pages = {1028--1034}, posted-at = {2006-07-19 11:05:29}, priority = {2}, title = {Pyruvate transport across the plasma membrane of the bloodstream form of Trypanosoma brucei is mediated by a facilitated diffusion carrier}, url = {http://dx.doi.org/10.1016/0006-291X(92)90694-G}, volume = {184}, year = {1992} }

TY - JOUR ID - citeulike:764230 L3 - citeulike-article-id:764230 N2 - The characteristics of pyruvate transport across the plasma membrane in the bloodstream form of Trypanosoma brucei were studied using [14C]pyruvate in combination with the silicone-oil centrifugation technique. We present evidence for the existence of a facilitated diffusion carrier in the plasma membrane of T. brucei which specifically mediates the translocation of pyruvate. The uptake of pyruvate followed saturation kinetics (Km 1.96 +/- 0.28 mM; Cmax 36.61 +/- 1.15 nmol pyruvate/30 sec.mg protein), after correction of the data for a nonsaturable diffusion component. The uptake of pyruvate was competitively inhibited by a number of (oxo)monocarboxylic acids, including pyruvate analogs and metabolically related substances, but not by L-lactate. The transport exhibited the phenomenon of transacceleration, indicative for the involvement of a facilitated diffusion carrier. The carrier is highly specific for pyruvate and differs from other known monocarboxylate carriers present in the mitochondrial and/or plasma membrane of other eukaryotic cells in that it does not transport L-lactate. IS - 2 JF - Biochemical and Biophysical Research Communications EP - 1034 TI - Pyruvate transport across the plasma membrane of the bloodstream form of Trypanosoma brucei is mediated by a facilitated diffusion carrier VL - 184 SP - 1028 KW - 14c KW - pyruvate-transport AU - Wiemer, Erik AU - Ter AU - Michels, Paul AU - Opperdoes, Fred PY - 1992/04/30/ UR - http://dx.doi.org/10.1016/0006-291X(92)90694-G ER -