Expression of the candidate MCT-1 oncogene in B- and T-cell lymphoid malignancies Export

@article{citeulike:782544, abstract = {Our laboratory has recently discovered a novel candidate oncogene, MCT-1, amplified in human T-cell lymphoma and mapped to chromosome Xq22-24. This region is amplified in a subset of primary B-cell non-Hodgkin lymphoma (NHL), suggesting that increased copy number of a gene(s) located in this region confers a growth advantage to some primary human lymphomas. We examined a diverse panel of lymphoid malignancies for the expression of MCT-1. We demonstrated that there are significantly increased levels of MCT-1 protein in a panel of T-cell lymphoid cell lines and in non-Hodgkin lymphoma cell lines. Furthermore, we identified a subset of primary diffuse large B-cell lymphomas that exhibited elevated levels of MCT-1 protein. Interestingly, all transformed follicular lymphomas in our study demonstrated elevated protein levels of MCT-1. There was no detectable MCT-1 protein in leukemic cells from patients with chronic lymphocytic leukemia or in any healthy lymphoid tissue examined. Lymphoid cell lines overexpressing MCT-1 exhibited increased growth rates and displayed increased protection against apoptosis induced by serum starvation when compared with matched controls. We found that MCT-1--overexpressing cells show constitutively higher levels of phosphorylated PKB/Akt protein, especially under serum starvation. Activation of survival pathways may be an additional function of the MCT-1 gene. Our data suggest that high levels of MCT-1 protein may be associated with a high-risk subset of lymphoid neoplasms and may further support the potential role of MCT-1 in promoting human lymphoid tumor development. (Blood. 2003;102: 297-302) 10.1182/blood-2002-11-3486}, author = {Shi, Bo and Hsu, Hsin-Ling and Evens, Andy M. and Gordon, Leo I. and Gartenhaus, Ronald B.}, citeulike-article-id = {782544}, citeulike-linkout-0 = {http://www.bloodjournal.org/cgi/content/abstract/102/1/297}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12637315}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12637315}, day = {1}, journal = {Blood}, keywords = {cancer, lymphoma, mct}, month = {July}, number = {1}, pages = {297--302}, posted-at = {2006-08-02 09:05:31}, priority = {2}, title = {Expression of the candidate MCT-1 oncogene in B- and T-cell lymphoid malignancies}, url = {http://www.bloodjournal.org/cgi/content/abstract/102/1/297}, volume = {102}, year = {2003} }

TY - JOUR ID - citeulike:782544 L3 - citeulike-article-id:782544 N2 - Our laboratory has recently discovered a novel candidate oncogene, MCT-1, amplified in human T-cell lymphoma and mapped to chromosome Xq22-24. This region is amplified in a subset of primary B-cell non-Hodgkin lymphoma (NHL), suggesting that increased copy number of a gene(s) located in this region confers a growth advantage to some primary human lymphomas. We examined a diverse panel of lymphoid malignancies for the expression of MCT-1. We demonstrated that there are significantly increased levels of MCT-1 protein in a panel of T-cell lymphoid cell lines and in non-Hodgkin lymphoma cell lines. Furthermore, we identified a subset of primary diffuse large B-cell lymphomas that exhibited elevated levels of MCT-1 protein. Interestingly, all transformed follicular lymphomas in our study demonstrated elevated protein levels of MCT-1. There was no detectable MCT-1 protein in leukemic cells from patients with chronic lymphocytic leukemia or in any healthy lymphoid tissue examined. Lymphoid cell lines overexpressing MCT-1 exhibited increased growth rates and displayed increased protection against apoptosis induced by serum starvation when compared with matched controls. We found that MCT-1--overexpressing cells show constitutively higher levels of phosphorylated PKB/Akt protein, especially under serum starvation. Activation of survival pathways may be an additional function of the MCT-1 gene. Our data suggest that high levels of MCT-1 protein may be associated with a high-risk subset of lymphoid neoplasms and may further support the potential role of MCT-1 in promoting human lymphoid tumor development. (Blood. 2003;102: 297-302) 10.1182/blood-2002-11-3486 IS - 1 JF - Blood EP - 302 TI - Expression of the candidate MCT-1 oncogene in B- and T-cell lymphoid malignancies VL - 102 SP - 297 KW - cancer KW - lymphoma KW - mct AU - Shi, Bo AU - Hsu, Hsin-Ling AU - Evens, Andy AU - Gordon, Leo AU - Gartenhaus, Ronald PY - 2003/07/01/ UR - http://www.bloodjournal.org/cgi/content/abstract/102/1/297 ER -