Induced instability of two Arabidopsis constitutive pathogen-response alleles. Export

@article{citeulike:1115460, abstract = {Paramutation is an example of a non-Mendelian-directed allelic interaction that results in the epigenetic alteration of one allele. We describe a paramutation-like interaction between two alleles, bal and cpr1-1 (constitutive expressor of PR genes 1), which map to a complex R-like gene cluster on Arabidopsis chromosome 4. Both alleles cause dwarfing and constitutive defense responses, similar to another dwarf variant, ssi1 (suppressor of SA-insensitivity 1). Previous work has demonstrated that the bal and ssi1 phenotypes are caused by overexpression of an R-like gene from the cluster, which activates an salicylic acid-dependent defense pathway. Here, we show that the cpr1-1 variant does not alter gene expression from the R-like gene cluster. The bal and cpr1-1 alleles did not complement each other in F(1) hybrids, but F(2) populations that segregated bal and cpr1-1 alleles contained plants with normal morphology at a frequency of 20\%. By using molecularly marked bal and cpr1-1 lines, we found that the majority of the normal phenotypes were correlated with inheritance of an altered cpr1-1 allele. Our observation that cpr1-1 is a metastable allele suggests that cpr1-1 is an epigenetic allele. The cpr1-1 allele is the third candidate epigenetic allele originating from this R-like gene cluster, making the region a possible hotspot of epigenetic variation.}, address = {Department of Biology, Washington University, One Brookings Drive, St. Louis, MO 63130, USA.}, author = {Stokes, T. L. and Richards, E. J.}, citeulike-article-id = {1115460}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.112040999}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/12032362}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=12032362}, day = {28}, doi = {10.1073/pnas.112040999}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {bal1, cpr1, ssi1}, month = {May}, number = {11}, pages = {7792--7796}, posted-at = {2007-02-21 01:24:25}, priority = {2}, title = {Induced instability of two Arabidopsis constitutive pathogen-response alleles.}, url = {http://dx.doi.org/10.1073/pnas.112040999}, volume = {99}, year = {2002} }

TY - JOUR ID - citeulike:1115460 L3 - citeulike-article-id:1115460 N2 - Paramutation is an example of a non-Mendelian-directed allelic interaction that results in the epigenetic alteration of one allele. We describe a paramutation-like interaction between two alleles, bal and cpr1-1 (constitutive expressor of PR genes 1), which map to a complex R-like gene cluster on Arabidopsis chromosome 4. Both alleles cause dwarfing and constitutive defense responses, similar to another dwarf variant, ssi1 (suppressor of SA-insensitivity 1). Previous work has demonstrated that the bal and ssi1 phenotypes are caused by overexpression of an R-like gene from the cluster, which activates an salicylic acid-dependent defense pathway. Here, we show that the cpr1-1 variant does not alter gene expression from the R-like gene cluster. The bal and cpr1-1 alleles did not complement each other in F(1) hybrids, but F(2) populations that segregated bal and cpr1-1 alleles contained plants with normal morphology at a frequency of 20%. By using molecularly marked bal and cpr1-1 lines, we found that the majority of the normal phenotypes were correlated with inheritance of an altered cpr1-1 allele. Our observation that cpr1-1 is a metastable allele suggests that cpr1-1 is an epigenetic allele. The cpr1-1 allele is the third candidate epigenetic allele originating from this R-like gene cluster, making the region a possible hotspot of epigenetic variation. IS - 11 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Department of Biology, Washington University, One Brookings Drive, St. Louis, MO 63130, USA. EP - 7796 TI - Induced instability of two Arabidopsis constitutive pathogen-response alleles. VL - 99 SP - 7792 KW - bal1 KW - cpr1 KW - ssi1 AU - Stokes, TL AU - Richards, EJ PY - 2002/05/28/ UR - http://dx.doi.org/10.1073/pnas.112040999 ER -