RNA-binding proteins that inhibit RNA virus infection. Export

@article{citeulike:1378139, abstract = {Arrays of >5,000 Saccharomyces cerevisiae proteins were screened to identify proteins that can preferentially bind a small RNA hairpin that contains a clamped adenine motif (CAM). A CAM is required for the replication of Brome Mosaic Virus (BMV), a plant-infecting RNA virus that can replicate in S. cerevisiae. Several hits were selected for further characterization in Nicotiana benthamiana. Pseudouridine Synthase 4 (Pus4) and the Actin Patch Protein 1 (App1) modestly reduced BMV genomic plus-strand RNA accumulation, but dramatically inhibited BMV systemic spread in plants. Pus4 also prevented the encapsidation of a BMV RNA in plants and the reassembly of BMV virions in vitro. These results demonstrate the feasibility of using proteome arrays to identify specific RNA-binding proteins for antiviral activities. Furthermore, the effects of Pus4 suggest that the CAM-containing RNA motif provides a regulatory link between RNA replication and encapsidation.}, address = {Department of Pharmacology and Molecular Sciences and High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.}, author = {Zhu, J. and Gopinath, K. and Murali, A. and Yi, G. and Hayward, S. D. and Zhu, H. and Kao, C.}, citeulike-article-id = {1378139}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0611617104}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17360619}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17360619}, day = {27}, doi = {10.1073/pnas.0611617104}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {bromo, hostfactor}, month = {February}, number = {9}, pages = {3129--3134}, posted-at = {2007-06-11 06:53:39}, priority = {2}, title = {RNA-binding proteins that inhibit RNA virus infection.}, url = {http://dx.doi.org/10.1073/pnas.0611617104}, volume = {104}, year = {2007} }

TY - JOUR ID - citeulike:1378139 L3 - citeulike-article-id:1378139 N2 - Arrays of >5,000 Saccharomyces cerevisiae proteins were screened to identify proteins that can preferentially bind a small RNA hairpin that contains a clamped adenine motif (CAM). A CAM is required for the replication of Brome Mosaic Virus (BMV), a plant-infecting RNA virus that can replicate in S. cerevisiae. Several hits were selected for further characterization in Nicotiana benthamiana. Pseudouridine Synthase 4 (Pus4) and the Actin Patch Protein 1 (App1) modestly reduced BMV genomic plus-strand RNA accumulation, but dramatically inhibited BMV systemic spread in plants. Pus4 also prevented the encapsidation of a BMV RNA in plants and the reassembly of BMV virions in vitro. These results demonstrate the feasibility of using proteome arrays to identify specific RNA-binding proteins for antiviral activities. Furthermore, the effects of Pus4 suggest that the CAM-containing RNA motif provides a regulatory link between RNA replication and encapsidation. IS - 9 JF - Proc Natl Acad Sci U S A SN - 0027-8424 AD - Department of Pharmacology and Molecular Sciences and High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. EP - 3134 TI - RNA-binding proteins that inhibit RNA virus infection. VL - 104 SP - 3129 KW - bromo KW - hostfactor AU - Zhu, J AU - Gopinath, K AU - Murali, A AU - Yi, G AU - Hayward, SD AU - Zhu, H AU - Kao, C PY - 2007/02/27/ UR - http://dx.doi.org/10.1073/pnas.0611617104 ER -