Post-transcriptional regulation of thyroid hormone receptor expression by cis-acting sequences and a naturally occurring antisense RNA. Export

@article{citeulike:2201572, abstract = {Thyroid hormone (T(3)) coordinates growth, differentiation, and metabolism by binding to nuclear thyroid hormone receptors (TRs). The TRalpha gene encodes T(3)-activated TRalpha1 (NR1A1a) as well as an antagonistic, non-T(3)-binding alternatively spliced product, TRalpha2 (NR1A1b). Thus, the TRalpha1/TRalpha2 ratio is a critical determinant of T(3) action. However, the mechanisms underlying this post-transcriptional regulation are unknown. We have identified a non-consensus, TRalpha2-specific 5' splice site and conserved intronic sequences as key determinants of TRalpha mRNA processing. In addition to these cis-acting elements, a novel regulatory feature is the orphan receptor RevErbAalpha (NR1D1) gene, which is transcribed from the opposite direction at the same locus and overlaps the TRalpha2 coding region. RevErbAalpha gene expression correlates with a high TRalpha1/TRalpha2 ratio in a number of tissues. Here we demonstrate that coexpression of RevErbAalpha and TRalpha regulates the TRalpha1/TRalpha2 ratio in intact cells. Thus, both cis- and trans-regulatory mechanisms contribute to cell-specific post-transcriptional regulation of TR gene expression and T(3) action.}, address = {Department of Biology, Marquette University, Milwaukee, Wisconsin 53201, USA.}, author = {Hastings, M. L. and Ingle, H. A. and Lazar, M. A. and Munroe, S. H.}, citeulike-article-id = {2201572}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10753970}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10753970}, day = {14}, issn = {0021-9258}, journal = {J Biol Chem}, keywords = {alternative, natsirna}, month = {April}, number = {15}, pages = {11507--11513}, posted-at = {2008-01-07 03:09:49}, priority = {2}, title = {Post-transcriptional regulation of thyroid hormone receptor expression by cis-acting sequences and a naturally occurring antisense RNA.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10753970}, volume = {275}, year = {2000} }

TY - JOUR ID - citeulike:2201572 L3 - citeulike-article-id:2201572 N2 - Thyroid hormone (T(3)) coordinates growth, differentiation, and metabolism by binding to nuclear thyroid hormone receptors (TRs). The TRalpha gene encodes T(3)-activated TRalpha1 (NR1A1a) as well as an antagonistic, non-T(3)-binding alternatively spliced product, TRalpha2 (NR1A1b). Thus, the TRalpha1/TRalpha2 ratio is a critical determinant of T(3) action. However, the mechanisms underlying this post-transcriptional regulation are unknown. We have identified a non-consensus, TRalpha2-specific 5' splice site and conserved intronic sequences as key determinants of TRalpha mRNA processing. In addition to these cis-acting elements, a novel regulatory feature is the orphan receptor RevErbAalpha (NR1D1) gene, which is transcribed from the opposite direction at the same locus and overlaps the TRalpha2 coding region. RevErbAalpha gene expression correlates with a high TRalpha1/TRalpha2 ratio in a number of tissues. Here we demonstrate that coexpression of RevErbAalpha and TRalpha regulates the TRalpha1/TRalpha2 ratio in intact cells. Thus, both cis- and trans-regulatory mechanisms contribute to cell-specific post-transcriptional regulation of TR gene expression and T(3) action. IS - 15 JF - J Biol Chem SN - 0021-9258 AD - Department of Biology, Marquette University, Milwaukee, Wisconsin 53201, USA. EP - 11513 TI - Post-transcriptional regulation of thyroid hormone receptor expression by cis-acting sequences and a naturally occurring antisense RNA. VL - 275 SP - 11507 KW - alternative KW - natsirna AU - Hastings, ML AU - Ingle, HA AU - Lazar, MA AU - Munroe, SH PY - 2000/04/14/ UR - http://view.ncbi.nlm.nih.gov/pubmed/10753970 ER -