Analysis of alternative transcripts of the flax L6 rust resistance gene. Export

@article{citeulike:2266050, abstract = {The L6 rust resistance gene from flax generates at least four transcript classes by alternative splicing of the third intron. The most abundant transcript class encodes a resistance protein containing domains that include nucleotide binding site motifs and a leucine-rich repeat region (NBS-LRR). The remaining three transcript classes encode truncated products which lack most of the C-terminal part of the protein containing the leucine-rich region (LRR). The four transcript classes occur in all plant organs examined and no induction of L6 expression was observed following infection of resistant plants with an avirulent rust strain expressing the corresponding A-L6 avirulence gene. Flax plants transgenic for an intronless L6 gene, incapable of encoding truncated resistance proteins by alternative splicing, expressed L6 resistance indistinguishable from that of the wild-type gene. Therefore, a definitive role for alternative transcripts and their predicted truncated products could not be assigned in the flax/flax rust system.}, address = {CSIRO Plant Industry, Canberra ACT, Australia. michaela@pi.csiro.au}, author = {Ayliffe, M. A. and Frost, D. V. and Finnegan, E. J. and Lawrence, G. J. and Anderson, P. A. and Ellis, J. G.}, citeulike-article-id = {2266050}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10097386}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10097386}, issn = {0960-7412}, journal = {Plant J}, keywords = {alternative, l, r}, month = {February}, number = {3}, pages = {287--292}, posted-at = {2008-01-21 06:34:19}, priority = {2}, title = {Analysis of alternative transcripts of the flax L6 rust resistance gene.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10097386}, volume = {17}, year = {1999} }

TY - JOUR ID - citeulike:2266050 L3 - citeulike-article-id:2266050 N2 - The L6 rust resistance gene from flax generates at least four transcript classes by alternative splicing of the third intron. The most abundant transcript class encodes a resistance protein containing domains that include nucleotide binding site motifs and a leucine-rich repeat region (NBS-LRR). The remaining three transcript classes encode truncated products which lack most of the C-terminal part of the protein containing the leucine-rich region (LRR). The four transcript classes occur in all plant organs examined and no induction of L6 expression was observed following infection of resistant plants with an avirulent rust strain expressing the corresponding A-L6 avirulence gene. Flax plants transgenic for an intronless L6 gene, incapable of encoding truncated resistance proteins by alternative splicing, expressed L6 resistance indistinguishable from that of the wild-type gene. Therefore, a definitive role for alternative transcripts and their predicted truncated products could not be assigned in the flax/flax rust system. IS - 3 JF - Plant J SN - 0960-7412 AD - CSIRO Plant Industry, Canberra ACT, Australia. michaela@pi.csiro.au EP - 292 TI - Analysis of alternative transcripts of the flax L6 rust resistance gene. VL - 17 SP - 287 KW - alternative KW - l KW - r AU - Ayliffe, MA AU - Frost, DV AU - Finnegan, EJ AU - Lawrence, GJ AU - Anderson, PA AU - Ellis, JG PY - 1999/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/10097386 ER -