Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region. Export

@article{citeulike:2266056, abstract = {The M rust resistance gene from flax was cloned after two separate approaches, an analysis of spontaneous M mutants with an L6 gene-derived DNA probe and tagging with the maize transposon Activator, independently identified the same gene. The gene encodes a protein of the nucleotide binding site leucine-rich repeat class and is related (86\% nucleotide identity) to the unlinked L6 rust resistance gene. In contrast to the L locus, which contains a single gene with multiple alleles, approximately 15 related genes occur at the complex M locus, with only one encoding the M resistance specificity. The M protein contains two direct repeats of 147 and 149 amino acids in the C-terminal part of the leucine-rich region. Three mutant alleles of M encoding a product containing a single repeat unit of 154 amino acids were isolated. The mutant DNA sequences probably occurred by unequal intragenic exchange in the coding region of the repeats. The recombinant alleles lost M resistance and gained no detectable new resistance specificity.}, address = {Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, Canberra, Australia.}, author = {Anderson, P. A. and Lawrence, G. J. and Morrish, B. C. and Ayliffe, M. A. and Finnegan, E. J. and Ellis, J. G.}, citeulike-article-id = {2266056}, citeulike-linkout-0 = {http://dx.doi.org/10.1105/tpc.9.4.641}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/9144966}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=9144966}, doi = {10.1105/tpc.9.4.641}, issn = {1040-4651}, journal = {Plant Cell}, keywords = {alternative, m, r}, month = {April}, number = {4}, pages = {641--651}, posted-at = {2008-01-21 06:36:17}, priority = {2}, title = {Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region.}, url = {http://dx.doi.org/10.1105/tpc.9.4.641}, volume = {9}, year = {1997} }

TY - JOUR ID - citeulike:2266056 L3 - citeulike-article-id:2266056 N2 - The M rust resistance gene from flax was cloned after two separate approaches, an analysis of spontaneous M mutants with an L6 gene-derived DNA probe and tagging with the maize transposon Activator, independently identified the same gene. The gene encodes a protein of the nucleotide binding site leucine-rich repeat class and is related (86% nucleotide identity) to the unlinked L6 rust resistance gene. In contrast to the L locus, which contains a single gene with multiple alleles, approximately 15 related genes occur at the complex M locus, with only one encoding the M resistance specificity. The M protein contains two direct repeats of 147 and 149 amino acids in the C-terminal part of the leucine-rich region. Three mutant alleles of M encoding a product containing a single repeat unit of 154 amino acids were isolated. The mutant DNA sequences probably occurred by unequal intragenic exchange in the coding region of the repeats. The recombinant alleles lost M resistance and gained no detectable new resistance specificity. IS - 4 JF - Plant Cell SN - 1040-4651 AD - Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry, Canberra, Australia. EP - 651 TI - Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region. VL - 9 SP - 641 KW - alternative KW - m KW - r AU - Anderson, PA AU - Lawrence, GJ AU - Morrish, BC AU - Ayliffe, MA AU - Finnegan, EJ AU - Ellis, JG PY - 1997/04// UR - http://dx.doi.org/10.1105/tpc.9.4.641 ER -