Regional Tumor Oximetry: 19F NMR Spectroscopy of Hexafluorobenzene Export

@article{HU98, abstract = {Purpose: An accurate method for monitoring oxygen tension (pO 2 ) of individual tumors could be valuable for optimizing treatment plans. We have recently shown that 19 F nuclear magnetic resonance (NMR) spin-lattice relaxometry of hexafluorobenzene (HFB) provides a highly sensitive indicator of tumor oxygenation. We have now refined the methodology to provide enhanced precision, and applied the method to investigate dynamic changes in tumor oxygenation. Methods and Materials: Dunning prostate adenocarcinoma R3327-AT1 was grown in the form of pedicles on the foreback of male Copenhagen rats. When the tumors reached 1 cm diameter, HFB (20 μl) was administered, either centrally or peripherally, by direct intratumoral (IT) injection. Local pO 2 was determined using pulse-burst saturation recovery (PBSR) 19 F NMR spectroscopy on the basis of the spin-lattice relaxation rate, R1. Results: Interrogation of the central region of tumors provided typical values in the range pO 2 = 1.4–6.4 mmHg, with a typical stability of ±2 mmHg over a period of 20 min, when rats breathed 33\% O 2 . Altering the inhaled gas to oxygen or carbogen (95\% O 2 /5\% CO 2 ) produced no significant change. In contrast, interrogation of tumor periphery indicated baseline pO 2 in the range 7.9–78.9 mmHg. Altering inspired gas produced significant changes ( p < 0.0001) with O 2 or carbogen, although the change was generally greater with carbogen. In each case, pO 2 returned to baseline within 16 min of returning the inhaled gas to baseline. Conclusion: We believe this method provides a valuable new approach with the requisite precision and accuracy to investigate tumor pO 2 .}, author = {Hunjan, S. and Mason, R. and Constantinescu, A. and Peschke, P. and Hahn, E. and Antich, P.}, citeulike-article-id = {4216998}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0360-3016(98)00020-0}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0360301698000200}, day = {01}, doi = {10.1016/S0360-3016(98)00020-0}, issn = {03603016}, journal = {International Journal of Radiation OncologyBiologyPhysics}, keywords = {19f-mri, 19fo, ardvarc, blood, hexafluorobenzene, hu98, substitutes}, month = {April}, number = {1}, pages = {161--171}, posted-at = {2009-03-25 09:49:21}, priority = {0}, title = {Regional Tumor Oximetry: 19F NMR Spectroscopy of Hexafluorobenzene}, url = {http://dx.doi.org/10.1016/S0360-3016(98)00020-0}, volume = {41}, year = {1998} }

TY - JOUR ID - HU98 L3 - citeulike-article-id:4216998 N2 - Purpose: An accurate method for monitoring oxygen tension (pO 2 ) of individual tumors could be valuable for optimizing treatment plans. We have recently shown that 19 F nuclear magnetic resonance (NMR) spin-lattice relaxometry of hexafluorobenzene (HFB) provides a highly sensitive indicator of tumor oxygenation. We have now refined the methodology to provide enhanced precision, and applied the method to investigate dynamic changes in tumor oxygenation. Methods and Materials: Dunning prostate adenocarcinoma R3327-AT1 was grown in the form of pedicles on the foreback of male Copenhagen rats. When the tumors reached 1 cm diameter, HFB (20 μl) was administered, either centrally or peripherally, by direct intratumoral (IT) injection. Local pO 2 was determined using pulse-burst saturation recovery (PBSR) 19 F NMR spectroscopy on the basis of the spin-lattice relaxation rate, R1. Results: Interrogation of the central region of tumors provided typical values in the range pO 2 = 1.4–6.4 mmHg, with a typical stability of ±2 mmHg over a period of 20 min, when rats breathed 33% O 2 . Altering the inhaled gas to oxygen or carbogen (95% O 2 /5% CO 2 ) produced no significant change. In contrast, interrogation of tumor periphery indicated baseline pO 2 in the range 7.9–78.9 mmHg. Altering inspired gas produced significant changes ( p < 0.0001) with O 2 or carbogen, although the change was generally greater with carbogen. In each case, pO 2 returned to baseline within 16 min of returning the inhaled gas to baseline. Conclusion: We believe this method provides a valuable new approach with the requisite precision and accuracy to investigate tumor pO 2 . IS - 1 JF - International Journal of Radiation OncologyBiologyPhysics SN - 03603016 EP - 171 TI - Regional Tumor Oximetry: 19F NMR Spectroscopy of Hexafluorobenzene VL - 41 SP - 161 KW - 19f-mri KW - 19fo KW - ardvarc KW - blood KW - hexafluorobenzene KW - hu98 KW - substitutes AU - Hunjan, S AU - Mason, R AU - Constantinescu, A AU - Peschke, P AU - Hahn, E AU - Antich, P PY - 1998/04/01/ UR - http://dx.doi.org/10.1016/S0360-3016(98)00020-0 ER -