Cerebrospinal Fluid Dehydroepiandrosterone Levels Are Correlated with Brain Dehydroepiandrosterone Levels, Elevated in Alzheimer's Disease, and Related to Neuropathological Disease Stage. Export

@article{citeulike:2801637, abstract = {Objective: It is currently unknown if cerebrospinal fluid (CSF) neurosteroid levels are related to brain neurosteroid levels in humans. CSF and brain dehydroepiandrosterone (DHEA) levels are elevated in patients with Alzheimer's disease (AD), but it is unclear if CSF DHEA levels are correlated with brain DHEA levels within the same subject cohort. We therefore determined DHEA and pregnenolone levels in AD patients (n = 25) and cognitively intact control subjects (n = 16) in both CSF and temporal cortex. Design: DHEA and pregnenolone levels were determined by gas chromatography/mass spectrometry preceded by high performance liquid chromatography. Frozen CSF and temporal cortex specimens were provided by the Alzheimer's Disease Research Center at Duke University Medical Center. Data were analyzed by Mann-Whitney U test statistic and Spearman correlational analyses. Results: CSF DHEA levels are positively correlated with temporal cortex DHEA levels (r = 0.59, P < 0.0001) and neuropathological disease stage (Braak and Braak) [r = 0.42, P = 0.007]. CSF pregnenolone levels are also positively correlated with temporal cortex pregnenolone levels (r = 0.57, P < 0.0001), and tend to be correlated with neuropathological disease stage (Braak) [r = 0.30, P = 0.06]. CSF DHEA levels are elevated (P = 0.032) and pregnenolone levels tend to be elevated (P = 0.10) in patients with AD compared to cognitively intact control subjects. Conclusions: These findings indicate that CSF DHEA and pregnenolone levels are correlated with temporal cortex brain levels of these neurosteroids, and that CSF DHEA is elevated in AD and related to neuropathological disease stage. Neurosteroids may thus be relevant to the pathophysiology of AD.}, address = {Durham Veterans Affairs Medical Center, Durham, NC; Duke University Medical Center, Department of Psychiatry and Behavioral Sciences, Durham, NC; Joseph and Kathleen Bryan Alzheimer's Disease Research Center, Duke University Medical Center, Durham, NC; Columbia University College of Physicians and Surgeons, Department of Psychiatry, New York, NY; Duke University Medical Center, Department of Surgery, Durham, NC.}, author = {Naylor, Jennifer C C. and Hulette, Christine M M. and Steffens, David C C. and Shampine, Lawrence J J. and Ervin, John F F. and Payne, Victoria M M. and Massing, Mark W W. and Kilts, Jason D D. and Strauss, Jennifer L L. and Calhoun, Patrick S S. and Calnaido, Rohana P P. and Blazer, Daniel G G. and Lieberman, Jeffrey A A. and Madison, Roger D D. and Marx, Christine E E.}, citeulike-article-id = {2801637}, citeulike-linkout-0 = {http://dx.doi.org/10.1210/jc.2007-1229}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18477662}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18477662}, day = {13}, doi = {10.1210/jc.2007-1229}, issn = {0021-972X}, journal = {The Journal of clinical endocrinology and metabolism}, month = {May}, posted-at = {2008-05-15 14:45:57}, priority = {2}, title = {Cerebrospinal Fluid Dehydroepiandrosterone Levels Are Correlated with Brain Dehydroepiandrosterone Levels, Elevated in Alzheimer's Disease, and Related to Neuropathological Disease Stage.}, url = {http://dx.doi.org/10.1210/jc.2007-1229}, year = {2008} }

TY - JOUR ID - citeulike:2801637 L3 - citeulike-article-id:2801637 N2 - Objective: It is currently unknown if cerebrospinal fluid (CSF) neurosteroid levels are related to brain neurosteroid levels in humans. CSF and brain dehydroepiandrosterone (DHEA) levels are elevated in patients with Alzheimer's disease (AD), but it is unclear if CSF DHEA levels are correlated with brain DHEA levels within the same subject cohort. We therefore determined DHEA and pregnenolone levels in AD patients (n = 25) and cognitively intact control subjects (n = 16) in both CSF and temporal cortex. Design: DHEA and pregnenolone levels were determined by gas chromatography/mass spectrometry preceded by high performance liquid chromatography. Frozen CSF and temporal cortex specimens were provided by the Alzheimer's Disease Research Center at Duke University Medical Center. Data were analyzed by Mann-Whitney U test statistic and Spearman correlational analyses. Results: CSF DHEA levels are positively correlated with temporal cortex DHEA levels (r = 0.59, P < 0.0001) and neuropathological disease stage (Braak and Braak) [r = 0.42, P = 0.007]. CSF pregnenolone levels are also positively correlated with temporal cortex pregnenolone levels (r = 0.57, P < 0.0001), and tend to be correlated with neuropathological disease stage (Braak) [r = 0.30, P = 0.06]. CSF DHEA levels are elevated (P = 0.032) and pregnenolone levels tend to be elevated (P = 0.10) in patients with AD compared to cognitively intact control subjects. Conclusions: These findings indicate that CSF DHEA and pregnenolone levels are correlated with temporal cortex brain levels of these neurosteroids, and that CSF DHEA is elevated in AD and related to neuropathological disease stage. Neurosteroids may thus be relevant to the pathophysiology of AD. JF - The Journal of clinical endocrinology and metabolism SN - 0021-972X AD - Durham Veterans Affairs Medical Center, Durham, NC; Duke University Medical Center, Department of Psychiatry and Behavioral Sciences, Durham, NC; Joseph and Kathleen Bryan Alzheimer's Disease Research Center, Duke University Medical Center, Durham, NC; Columbia University College of Physicians and Surgeons, Department of Psychiatry, New York, NY; Duke University Medical Center, Department of Surgery, Durham, NC. TI - Cerebrospinal Fluid Dehydroepiandrosterone Levels Are Correlated with Brain Dehydroepiandrosterone Levels, Elevated in Alzheimer's Disease, and Related to Neuropathological Disease Stage. AU - Naylor, Jennifer C AU - Hulette, Christine M AU - Steffens, David C AU - Shampine, Lawrence J AU - Ervin, John F AU - Payne, Victoria M AU - Massing, Mark W AU - Kilts, Jason D AU - Strauss, Jennifer L AU - Calhoun, Patrick S AU - Calnaido, Rohana P AU - Blazer, Daniel G AU - Lieberman, Jeffrey A AU - Madison, Roger D AU - Marx, Christine E PY - 2008/05/13/ UR - http://dx.doi.org/10.1210/jc.2007-1229 ER -