Discovery of neurosteroid glucuronides in mouse brain. Export

@article{citeulike:5743329, abstract = {Neurosteroid glucuronides were found for the first time in brain samples. The intact glucuronides were extracted from the cortex, hippocampus, hypothalamus, and mid-brain tissues of nicotine- and water-treated mice, and detected with capillary liquid chromatography-electrospray-tandem mass spectrometry (CapLC-ESI-MS/MS). The glucuronides of estradiol, cortisol, corticosterone, tetrahydrodeoxycorticosterone, pregnenolone, and isopregnanolone were identified by comparing retention times in selected reaction monitoring (SRM) chromatograms and the relative abundances of two SRM transitions of each neurosteroid glucuronide between the reference and authentic samples, thus providing reliable identification. In vitro experiments, carried out by using S9 fractions from mouse and rat brains, showed a formation of glucuronides with selected test compounds (corticosterone, pregnenolone, and dehydroepiandrosterone), suggesting that biosynthesis of neurosteroid glucuronides is possible in rodent brain.}, author = {Kallonen, Sirkku E. and Tammim\"{a}ki, Anne and Piepponen, Petteri and Raattamaa, Helena and Ketola, Raimo A. and Kostiainen, Risto}, citeulike-article-id = {5743329}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.aca.2009.07.059}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19733737}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19733737}, day = {28}, doi = {10.1016/j.aca.2009.07.059}, issn = {1873-4324}, journal = {Analytica chimica acta}, keywords = {iliad}, month = {September}, number = {1}, pages = {69--74}, posted-at = {2009-09-08 15:23:16}, priority = {2}, title = {Discovery of neurosteroid glucuronides in mouse brain.}, url = {http://dx.doi.org/10.1016/j.aca.2009.07.059}, volume = {651}, year = {2009} }

TY - JOUR ID - citeulike:5743329 L3 - citeulike-article-id:5743329 N2 - Neurosteroid glucuronides were found for the first time in brain samples. The intact glucuronides were extracted from the cortex, hippocampus, hypothalamus, and mid-brain tissues of nicotine- and water-treated mice, and detected with capillary liquid chromatography-electrospray-tandem mass spectrometry (CapLC-ESI-MS/MS). The glucuronides of estradiol, cortisol, corticosterone, tetrahydrodeoxycorticosterone, pregnenolone, and isopregnanolone were identified by comparing retention times in selected reaction monitoring (SRM) chromatograms and the relative abundances of two SRM transitions of each neurosteroid glucuronide between the reference and authentic samples, thus providing reliable identification. In vitro experiments, carried out by using S9 fractions from mouse and rat brains, showed a formation of glucuronides with selected test compounds (corticosterone, pregnenolone, and dehydroepiandrosterone), suggesting that biosynthesis of neurosteroid glucuronides is possible in rodent brain. IS - 1 JF - Analytica chimica acta SN - 1873-4324 EP - 74 TI - Discovery of neurosteroid glucuronides in mouse brain. VL - 651 SP - 69 KW - iliad AU - Kallonen, Sirkku AU - Tammimäki, Anne AU - Piepponen, Petteri AU - Raattamaa, Helena AU - Ketola, Raimo AU - Kostiainen, Risto PY - 2009/09/28/ UR - http://dx.doi.org/10.1016/j.aca.2009.07.059 ER -