A Genome-scale Metabolic Model of Arabidopsis thaliana and Some of its Properties. Export

@article{citeulike:6070577, abstract = {We describe the construction and analysis of a genome-scale metabolic model of Arabidopsis thaliana primarily derived from the annotations in the Aracyc database. We used techniques based on Linear Programming to demonstrate that: 1) the model is capable of producing biomass components (amino-acids, nucleotides, lipid, starch and cellulose) in the proportion observed experimentally in a heterotrophic suspension culture ; 2) That, approximately, only 15 \% of the available reactions are needed for this purpose and that the size of this network is comparable to estimates of minimal network size for other organisms ; 3) That reactions may be grouped according to the changes in flux resulting from a hypothetical stimulus (in this case demand for ATP), and that this allows the identification of potential metabolic modules ; 4) That total ATP demand for growth and maintenance can be inferred, and that this is consistent with previous estimates in prokaryotes and yeast.}, author = {Poolman, Mark G. and Miguet, Laurent and Sweetlove, Lee J. and Fell, David A.}, citeulike-article-id = {6070577}, citeulike-linkout-0 = {http://dx.doi.org/10.1104/pp.109.141267}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19755544}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19755544}, day = {15}, doi = {10.1104/pp.109.141267}, issn = {0032-0889}, journal = {Plant physiology}, keywords = {arabidopsis, metabolic\_reconstruction, plant}, month = {September}, posted-at = {2009-11-04 17:18:55}, priority = {2}, title = {A Genome-scale Metabolic Model of Arabidopsis thaliana and Some of its Properties.}, url = {http://dx.doi.org/10.1104/pp.109.141267}, year = {2009} }

TY - JOUR ID - citeulike:6070577 L3 - citeulike-article-id:6070577 N2 - We describe the construction and analysis of a genome-scale metabolic model of Arabidopsis thaliana primarily derived from the annotations in the Aracyc database. We used techniques based on Linear Programming to demonstrate that: 1) the model is capable of producing biomass components (amino-acids, nucleotides, lipid, starch and cellulose) in the proportion observed experimentally in a heterotrophic suspension culture ; 2) That, approximately, only 15 % of the available reactions are needed for this purpose and that the size of this network is comparable to estimates of minimal network size for other organisms ; 3) That reactions may be grouped according to the changes in flux resulting from a hypothetical stimulus (in this case demand for ATP), and that this allows the identification of potential metabolic modules ; 4) That total ATP demand for growth and maintenance can be inferred, and that this is consistent with previous estimates in prokaryotes and yeast. JF - Plant physiology SN - 0032-0889 TI - A Genome-scale Metabolic Model of Arabidopsis thaliana and Some of its Properties. KW - arabidopsis KW - metabolic_reconstruction KW - plant AU - Poolman, Mark AU - Miguet, Laurent AU - Sweetlove, Lee AU - Fell, David PY - 2009/09/15/ UR - http://dx.doi.org/10.1104/pp.109.141267 ER -