A surface transporter family conveys the trypanosome differentiation signal Export

@article{citeulike:4521629, abstract = {Microbial pathogens use environmental cues to trigger the developmental events needed to infect mammalian hosts or transmit to disease vectors. The parasites causing African sleeping sickness respond to citrate or cis-aconitate (CCA) to initiate life-cycle development when transmitted to their tsetse fly vector. This requires hypersensitization of the parasites to CCA by exposure to low temperature, conditions encountered after tsetse fly feeding at dusk or dawn. Here we identify a carboxylate-transporter family, PAD (proteins associated with differentiation), required for perception of this differentiation signal. Consistent with predictions for the response of trypanosomes to CCA, PAD proteins are expressed on the surface of the transmission-competent 'stumpy-form' parasites in the bloodstream, and at least one member is thermoregulated, showing elevated expression and surface access at low temperature. Moreover, RNA-interference-mediated ablation of PAD expression diminishes CCA-induced differentiation and eliminates CCA hypersensitivity under cold-shock conditions. As well as being molecular transducers of the differentiation signal in these parasites, PAD proteins provide the first example of a surface marker able to discriminate the transmission stage of trypanosomes in their mammalian host.}, author = {Dean, Samuel and Marchetti, Rosa and Kirk, Kiaran and Matthews, Keith R.}, citeulike-article-id = {4521629}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature07997}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature07997}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19444208}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19444208}, day = {14}, doi = {10.1038/nature07997}, issn = {0028-0836}, journal = {Nature}, keywords = {brucei, kinetoplastid, pad1, parasite, stumpy, trypanosoma}, month = {May}, number = {7244}, pages = {213--217}, posted-at = {2009-10-01 00:02:30}, priority = {0}, publisher = {Nature Publishing Group}, title = {A surface transporter family conveys the trypanosome differentiation signal}, url = {http://dx.doi.org/10.1038/nature07997}, volume = {459}, year = {2009} }

TY - JOUR ID - citeulike:4521629 L3 - citeulike-article-id:4521629 N2 - Microbial pathogens use environmental cues to trigger the developmental events needed to infect mammalian hosts or transmit to disease vectors. The parasites causing African sleeping sickness respond to citrate or cis-aconitate (CCA) to initiate life-cycle development when transmitted to their tsetse fly vector. This requires hypersensitization of the parasites to CCA by exposure to low temperature, conditions encountered after tsetse fly feeding at dusk or dawn. Here we identify a carboxylate-transporter family, PAD (proteins associated with differentiation), required for perception of this differentiation signal. Consistent with predictions for the response of trypanosomes to CCA, PAD proteins are expressed on the surface of the transmission-competent 'stumpy-form' parasites in the bloodstream, and at least one member is thermoregulated, showing elevated expression and surface access at low temperature. Moreover, RNA-interference-mediated ablation of PAD expression diminishes CCA-induced differentiation and eliminates CCA hypersensitivity under cold-shock conditions. As well as being molecular transducers of the differentiation signal in these parasites, PAD proteins provide the first example of a surface marker able to discriminate the transmission stage of trypanosomes in their mammalian host. IS - 7244 JF - Nature SN - 0028-0836 EP - 217 TI - A surface transporter family conveys the trypanosome differentiation signal PB - Nature Publishing Group VL - 459 SP - 213 KW - brucei KW - kinetoplastid KW - pad1 KW - parasite KW - stumpy KW - trypanosoma AU - Dean, Samuel AU - Marchetti, Rosa AU - Kirk, Kiaran AU - Matthews, Keith PY - 2009/05/14/ UR - http://dx.doi.org/10.1038/nature07997 ER -