Next-generation DNA sequencing Export

@article{citeulike:3391364, abstract = {DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection. Over the past three years, massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and democratizing the field by putting the sequencing capacity of a major genome center in the hands of individual investigators. These new technologies are rapidly evolving, and near-term challenges include the development of robust protocols for generating sequencing libraries, building effective new approaches to data-analysis, and often a rethinking of experimental design. Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes, transcriptomes and interactomes to become inexpensive, routine and widespread, rather than requiring significant production-scale efforts.}, author = {Shendure, Jay and Ji, Hanlee}, booktitle = {Nat Biotech}, citeulike-article-id = {3391364}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt1486}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt1486}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18846087}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18846087}, day = {09}, doi = {10.1038/nbt1486}, issn = {1087-0156}, journal = {Nature Biotechnology}, keywords = {next-generation, sequencing}, month = {October}, number = {10}, pages = {1135--1145}, posted-at = {2009-10-28 18:07:46}, priority = {2}, publisher = {Nature Publishing Group}, title = {Next-generation DNA sequencing}, url = {http://dx.doi.org/10.1038/nbt1486}, volume = {26}, year = {2008} }

TY - JOUR ID - citeulike:3391364 L3 - citeulike-article-id:3391364 N2 - DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection. Over the past three years, massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and democratizing the field by putting the sequencing capacity of a major genome center in the hands of individual investigators. These new technologies are rapidly evolving, and near-term challenges include the development of robust protocols for generating sequencing libraries, building effective new approaches to data-analysis, and often a rethinking of experimental design. Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes, transcriptomes and interactomes to become inexpensive, routine and widespread, rather than requiring significant production-scale efforts. IS - 10 JF - Nature Biotechnology SN - 1087-0156 EP - 1145 TI - Next-generation DNA sequencing PB - Nature Publishing Group VL - 26 T2 - Nat Biotech SP - 1135 KW - next-generation KW - sequencing AU - Shendure, Jay AU - Ji, Hanlee PY - 2008/10/09/ UR - http://dx.doi.org/10.1038/nbt1486 ER -