Critical DNA binding interactions of the insulator protein CTCF: a small number of zinc fingers mediate strong binding, and a single finger-DNA interaction controls binding at imprinted loci. Export

@article{citeulike:2447726, abstract = {The DNA-binding protein CTCF (CCCTC binding factor) mediates enhancer blocking insulation at sites throughout the genome and plays an important role in regulating allele-specific expression at the Igf2/H19 locus and at other imprinted loci. Evidence is also accumulating that CTCF is involved in large scale organization of genomic chromatin. Although CTCF has 11 zinc fingers, we show here that only 4 of these are essential to strong binding and that they recognize a core 12-bp DNA sequence common to most CTCF sites. By deleting individual fingers and mutating individual sites, we determined the orientation of binding. Furthermore, we were able to identify the specific finger and its point of DNA interaction that are responsible for the loss of CTCF binding when CpG residues are methylated in the imprinted Igf2/H19 locus. This single interaction appears to be critical for allele-specific binding and insulation by CTCF.}, address = {Dipartimento di Scienze Ambientali, Seconda Universit\`{a} degli Studi di Napoli via Vivaldi 43, 81100 Caserta, Italy.}, author = {Renda, M. and Baglivo, I. and Burgess-Beusse, B. and Esposito, S. and Fattorusso, R. and Felsenfeld, G. and Pedone, P. V.}, citeulike-article-id = {2447726}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M706213200}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17827499}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17827499}, day = {16}, doi = {10.1074/jbc.M706213200}, issn = {0021-9258}, journal = {The Journal of biological chemistry}, keywords = {affinity, assymetry, binding, binding-site, cpg, cpg-methylation, ctcf, dna-methylation, h19, igf2, methylation, orientation, zing-finger}, month = {November}, number = {46}, pages = {33336--33345}, posted-at = {2009-06-18 23:25:55}, priority = {0}, title = {Critical DNA binding interactions of the insulator protein CTCF: a small number of zinc fingers mediate strong binding, and a single finger-DNA interaction controls binding at imprinted loci.}, url = {http://dx.doi.org/10.1074/jbc.M706213200}, volume = {282}, year = {2007} }

TY - JOUR ID - citeulike:2447726 L3 - citeulike-article-id:2447726 N2 - The DNA-binding protein CTCF (CCCTC binding factor) mediates enhancer blocking insulation at sites throughout the genome and plays an important role in regulating allele-specific expression at the Igf2/H19 locus and at other imprinted loci. Evidence is also accumulating that CTCF is involved in large scale organization of genomic chromatin. Although CTCF has 11 zinc fingers, we show here that only 4 of these are essential to strong binding and that they recognize a core 12-bp DNA sequence common to most CTCF sites. By deleting individual fingers and mutating individual sites, we determined the orientation of binding. Furthermore, we were able to identify the specific finger and its point of DNA interaction that are responsible for the loss of CTCF binding when CpG residues are methylated in the imprinted Igf2/H19 locus. This single interaction appears to be critical for allele-specific binding and insulation by CTCF. IS - 46 JF - The Journal of biological chemistry SN - 0021-9258 AD - Dipartimento di Scienze Ambientali, Seconda Università degli Studi di Napoli via Vivaldi 43, 81100 Caserta, Italy. EP - 33345 TI - Critical DNA binding interactions of the insulator protein CTCF: a small number of zinc fingers mediate strong binding, and a single finger-DNA interaction controls binding at imprinted loci. VL - 282 SP - 33336 KW - affinity KW - assymetry KW - binding KW - binding-site KW - cpg KW - cpg-methylation KW - ctcf KW - dna-methylation KW - h19 KW - igf2 KW - methylation KW - orientation KW - zing-finger AU - Renda, M AU - Baglivo, I AU - Burgess-Beusse, B AU - Esposito, S AU - Fattorusso, R AU - Felsenfeld, G AU - Pedone, PV PY - 2007/11/16/ UR - http://dx.doi.org/10.1074/jbc.M706213200 ER -