Nuclear architecture and chromatin dynamics revealed by atomic force microscopy in combination with biochemistry and cell biology. Export

@article{citeulike:2793529, abstract = {The recent technical development of atomic force microscopy (AFM) has made nano-biology of the nucleus an attractive and promising field. In this paper, we will review our current understanding of nuclear architecture and dynamics from the structural point of view. Especially, special emphases will be given to: (1) How to approach the nuclear architectures by means of new techniques using AFM, (2) the importance of the physical property of DNA in the construction of the higher-order structures, (3) the significance and implication of the linker and core histones and the nuclear matrix/scaffold proteins for the chromatin dynamics, (4) the nuclear proteins that contribute to the formation of the inner nuclear architecture. Spatio-temporal analyses using AFM, in combination with biochemical and cell biological approaches, will play important roles in the nano-biology of the nucleus, as most of nuclear structures and events occur in nanometer, piconewton and millisecond order. The new applications of AFM, such as recognition imaging, fast-scanning imaging, and a variety of modified cantilevers, are expected to be powerful techniques to reveal the nanostructure of the nucleus.}, author = {Hirano, Yasuhiro and Takahashi, Hirohide and Kumeta, Masahiro and Hizume, Kohji and Hirai, Yuya and Otsuka, Shotaro and Yoshimura, Shige H. and Takeyasu, Kunio}, citeulike-article-id = {2793529}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s00424-007-0431-z}, citeulike-linkout-1 = {http://www.ingentaconnect.com/content/klu/424/2008/00000456/00000001/00000431}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18172599}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18172599}, doi = {10.1007/s00424-007-0431-z}, issn = {0031-6768}, journal = {Pfl\"{u}gers Archiv : European journal of physiology}, keywords = {afm, aml, atomic-force-microscopy, chromatin, mucleolus, nuclear-architecture, nucleus, specke}, month = {April}, number = {1}, pages = {139--153}, posted-at = {2009-10-26 18:43:24}, priority = {0}, publisher = {Springer}, title = {Nuclear architecture and chromatin dynamics revealed by atomic force microscopy in combination with biochemistry and cell biology.}, url = {http://dx.doi.org/10.1007/s00424-007-0431-z}, volume = {456}, year = {2008} }

TY - JOUR ID - citeulike:2793529 L3 - citeulike-article-id:2793529 N2 - The recent technical development of atomic force microscopy (AFM) has made nano-biology of the nucleus an attractive and promising field. In this paper, we will review our current understanding of nuclear architecture and dynamics from the structural point of view. Especially, special emphases will be given to: (1) How to approach the nuclear architectures by means of new techniques using AFM, (2) the importance of the physical property of DNA in the construction of the higher-order structures, (3) the significance and implication of the linker and core histones and the nuclear matrix/scaffold proteins for the chromatin dynamics, (4) the nuclear proteins that contribute to the formation of the inner nuclear architecture. Spatio-temporal analyses using AFM, in combination with biochemical and cell biological approaches, will play important roles in the nano-biology of the nucleus, as most of nuclear structures and events occur in nanometer, piconewton and millisecond order. The new applications of AFM, such as recognition imaging, fast-scanning imaging, and a variety of modified cantilevers, are expected to be powerful techniques to reveal the nanostructure of the nucleus. IS - 1 JF - Pflügers Archiv : European journal of physiology SN - 0031-6768 EP - 153 TI - Nuclear architecture and chromatin dynamics revealed by atomic force microscopy in combination with biochemistry and cell biology. PB - Springer VL - 456 SP - 139 KW - afm KW - aml KW - atomic-force-microscopy KW - chromatin KW - mucleolus KW - nuclear-architecture KW - nucleus KW - specke AU - Hirano, Yasuhiro AU - Takahashi, Hirohide AU - Kumeta, Masahiro AU - Hizume, Kohji AU - Hirai, Yuya AU - Otsuka, Shotaro AU - Yoshimura, Shige AU - Takeyasu, Kunio PY - 2008/04// UR - http://dx.doi.org/10.1007/s00424-007-0431-z ER -