Paternal imprints can be established on the maternal Igf2-H19 locus without altering replication timing of DNA. Export

@article{citeulike:2999101, abstract = {Genomic imprinting in mammals marks the parental alleles in gametes, resulting in differential gene expression in offspring. A number of epigenetic features are associated with imprinted genes. These include differential DNA methylation, histone acetylation and methylation, subnuclear localization and DNA replication timing. While DNA methylation has been shown to be necessary both for establishment and maintenance of imprinting, the connections with the other types of epigenetic marking systems are not clear. Specifically, it is not known whether the other marking systems, either on their own or in conjunction with DNA methylation, are required for imprinting. Here we show that in the mouse mutant Minute (Mnt) the Igf2-H19 locus acquires a paternal methylation imprint in the maternal germline. DNA methylation of the H19 DMR is established in oogenesis, maintained during postzygotic development on the maternal allele, and erased in primordial germ cells. The fact that a paternal type methylation imprint can also be established in the maternal germline indicates that trans-acting factors that target methylation to this imprinted region are likely to be the same in both germlines. Surprisingly, however, asynchrony of DNA replication of the locus is maintained despite the altered expression and methylation imprint of Igf2 and H19. These results show clearly that replication asynchrony of this region is neither the determinant factor for, nor a consequence of, epigenetic modifications that are critical for genomic imprinting. Replication asynchrony may thus be regulated differently from methylation imprints and have a separate function.}, address = {Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, UK.}, author = {Cerrato, F. and Dean, W. and Davies, K. and Kagotani, K. and Mitsuya, K. and Okumura, K. and Riccio, A. and Reik, W.}, citeulike-article-id = {2999101}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/14532328}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=14532328}, day = {1}, issn = {0964-6906}, journal = {Human molecular genetics}, keywords = {h19, igf2, imprinting, replication}, month = {December}, number = {23}, pages = {3123--3132}, posted-at = {2008-07-14 16:18:25}, priority = {0}, title = {Paternal imprints can be established on the maternal Igf2-H19 locus without altering replication timing of DNA.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/14532328}, volume = {12}, year = {2003} }

TY - JOUR ID - citeulike:2999101 L3 - citeulike-article-id:2999101 N2 - Genomic imprinting in mammals marks the parental alleles in gametes, resulting in differential gene expression in offspring. A number of epigenetic features are associated with imprinted genes. These include differential DNA methylation, histone acetylation and methylation, subnuclear localization and DNA replication timing. While DNA methylation has been shown to be necessary both for establishment and maintenance of imprinting, the connections with the other types of epigenetic marking systems are not clear. Specifically, it is not known whether the other marking systems, either on their own or in conjunction with DNA methylation, are required for imprinting. Here we show that in the mouse mutant Minute (Mnt) the Igf2-H19 locus acquires a paternal methylation imprint in the maternal germline. DNA methylation of the H19 DMR is established in oogenesis, maintained during postzygotic development on the maternal allele, and erased in primordial germ cells. The fact that a paternal type methylation imprint can also be established in the maternal germline indicates that trans-acting factors that target methylation to this imprinted region are likely to be the same in both germlines. Surprisingly, however, asynchrony of DNA replication of the locus is maintained despite the altered expression and methylation imprint of Igf2 and H19. These results show clearly that replication asynchrony of this region is neither the determinant factor for, nor a consequence of, epigenetic modifications that are critical for genomic imprinting. Replication asynchrony may thus be regulated differently from methylation imprints and have a separate function. IS - 23 JF - Human molecular genetics SN - 0964-6906 AD - Laboratory of Developmental Genetics and Imprinting, The Babraham Institute, Cambridge, UK. EP - 3132 TI - Paternal imprints can be established on the maternal Igf2-H19 locus without altering replication timing of DNA. VL - 12 SP - 3123 KW - h19 KW - igf2 KW - imprinting KW - replication AU - Cerrato, F AU - Dean, W AU - Davies, K AU - Kagotani, K AU - Mitsuya, K AU - Okumura, K AU - Riccio, A AU - Reik, W PY - 2003/12/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/14532328 ER -