Asynchronous replication of imprinted genes is established in the gametes and maintained during development.

@article{citeulike:3015186, abstract = {Genomic imprinting is characterized by allele-specific expression of multiple genes within large chromosomal domains that undergo DNA replication asynchronously during S phase. Here we show, using both fluorescence in situ hybridization analysis and S-phase fractionation techniques, that differential replication timing is associated with imprinted genes in a variety of cell types, and is already present in the pre-implantation embryo soon after fertilization. This pattern is erased before meiosis in the germ line, and parent-specific replication timing is then reset in late gametogenesis in both the male and female. Thus, asynchronous replication timing is established in the gametes and maintained throughout development, indicating that it may function as a primary epigenetic marker for distinguishing between the parental alleles.}, address = {Department of Cellular Biochemistry, Hebrew University, Jerusalem, Israel.}, author = {Simon, I. and Tenzen, T. and Reubinoff, B. E. and Hillman, D. and McCarrey, J. R. and Cedar, H.}, citeulike-article-id = {3015186}, doi = {10.1038/44866}, issn = {0028-0836}, journal = {Nature}, keywords = {development, gamete, imprinting, replication}, month = {October}, number = {6756}, pages = {929--932}, posted-at = {2008-07-17 18:39:02}, priority = {0}, title = {Asynchronous replication of imprinted genes is established in the gametes and maintained during development.}, url = {http://dx.doi.org/10.1038/44866}, volume = {401}, year = {1999} }

TY - JOUR ID - citeulike:3015186 L3 - citeulike-article-id:3015186 N2 - Genomic imprinting is characterized by allele-specific expression of multiple genes within large chromosomal domains that undergo DNA replication asynchronously during S phase. Here we show, using both fluorescence in situ hybridization analysis and S-phase fractionation techniques, that differential replication timing is associated with imprinted genes in a variety of cell types, and is already present in the pre-implantation embryo soon after fertilization. This pattern is erased before meiosis in the germ line, and parent-specific replication timing is then reset in late gametogenesis in both the male and female. Thus, asynchronous replication timing is established in the gametes and maintained throughout development, indicating that it may function as a primary epigenetic marker for distinguishing between the parental alleles. IS - 6756 JF - Nature SN - 0028-0836 AD - Department of Cellular Biochemistry, Hebrew University, Jerusalem, Israel. EP - 932 TI - Asynchronous replication of imprinted genes is established in the gametes and maintained during development. VL - 401 SP - 929 KW - development KW - gamete KW - imprinting KW - replication AU - Simon, I AU - Tenzen, T AU - Reubinoff, BE AU - Hillman, D AU - McCarrey, JR AU - Cedar, H PY - 1999/10/28/ UR - http://dx.doi.org/10.1038/44866 ER -