Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation. Export

@article{citeulike:3268975, abstract = {The loxP/Cre recombination system is a widely used tool for mouse functional genomics, in particular for in vivo conditional mutagenesis. Depending on the relative orientation and position of loxP sites, Cre-mediated recombination can result in a variety of targeted genomic rearrangements. It was previously reported that loss of the loxP-carrying chromosome can occur when loxP sites are arranged in inverse orientation. By using a chromosome 2 carrying inverted loxP sites, we found that Cre-mediated recombination not only causes chromosomal loss but also triggers apoptosis. We show that targeted recombination between inverted loxP sites (TRIP) triggers cell death specifically in proliferating Cre-expressing cells, and we provide evidence that TRIP is an efficient tool to ablate proliferating cells within genetically defined cell populations. Furthermore, the procedure requires only a simple, one-step intercross but neither the use of toxins nor the additional step of prodrug injection. With the large repertoire of tissue-specific or inducible Cre-expressing transgenes available, TRIP-mediated cell ablation is valuable to investigate the function of a large variety of cell populations in the context of a whole organism, which includes mechanisms underlying organ development and tissue homeostasis.}, address = {Laboratory of Genetics and Development, Institut de Recherches Cliniques de Montr\'{e}al (IRCM), 110 Avenue des Pins Ouest, H2W1R7, Universit\'{e} de Montr\'{e}al, Montr\'{e}al Qu\'{e}bec, Canada.}, author = {Gr\'{e}goire, Damien and Kmita, Marie}, citeulike-article-id = {3268975}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0807484105}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18787116}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18787116}, day = {11}, doi = {10.1073/pnas.0807484105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {ablation, apoptosis, cre, lox}, month = {September}, posted-at = {2008-09-15 13:31:43}, priority = {2}, title = {Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.}, url = {http://dx.doi.org/10.1073/pnas.0807484105}, year = {2008} }

TY - JOUR ID - citeulike:3268975 L3 - citeulike-article-id:3268975 N2 - The loxP/Cre recombination system is a widely used tool for mouse functional genomics, in particular for in vivo conditional mutagenesis. Depending on the relative orientation and position of loxP sites, Cre-mediated recombination can result in a variety of targeted genomic rearrangements. It was previously reported that loss of the loxP-carrying chromosome can occur when loxP sites are arranged in inverse orientation. By using a chromosome 2 carrying inverted loxP sites, we found that Cre-mediated recombination not only causes chromosomal loss but also triggers apoptosis. We show that targeted recombination between inverted loxP sites (TRIP) triggers cell death specifically in proliferating Cre-expressing cells, and we provide evidence that TRIP is an efficient tool to ablate proliferating cells within genetically defined cell populations. Furthermore, the procedure requires only a simple, one-step intercross but neither the use of toxins nor the additional step of prodrug injection. With the large repertoire of tissue-specific or inducible Cre-expressing transgenes available, TRIP-mediated cell ablation is valuable to investigate the function of a large variety of cell populations in the context of a whole organism, which includes mechanisms underlying organ development and tissue homeostasis. JF - Proceedings of the National Academy of Sciences of the United States of America SN - 1091-6490 AD - Laboratory of Genetics and Development, Institut de Recherches Cliniques de Montréal (IRCM), 110 Avenue des Pins Ouest, H2W1R7, Université de Montréal, Montréal Québec, Canada. TI - Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation. KW - ablation KW - apoptosis KW - cre KW - lox AU - Grégoire, Damien AU - Kmita, Marie PY - 2008/09/11/ UR - http://dx.doi.org/10.1073/pnas.0807484105 ER -