Differential effect of zinc finger deletions on the binding of CTCF to the promoter of the amyloid precursor protein gene. Export

@article{citeulike:4895977, abstract = {High levels of transcription from the amyloid precursor protein promoter are dependent on the binding of CTCF to the APBbeta core recognition sequence located between positions -82 and -93 upstream from the transcriptional start site. CTCF comprises 727 amino acids and contains 11 zinc finger motifs arranged in tandem that are flanked by 267 amino acids on the N-terminal side and 150 amino acids on the C-terminal side. Deletion of either the N- or the C-terminal regions outside of the zinc finger domain had no detrimental effect on the binding of CTCF to APBbeta. However, internal deletions of zinc fingers 5-7 completely abolished binding. The binding of full-length CTCF generated a DNase I protected domain extending from position -78 to -116, which was interrupted by a hypersensitive site at position -99. Selective deletions from the N- and C-terminal sides of the zinc finger domain showed that the N-terminal end of the zinc finger domain was aligned toward the transcriptional start site. Furthermore, deletions of zinc fingers peripheral to the essential zinc fingers 5-7 decreased the stability of the binding complex by interrupting sequence-specific interactions.}, author = {Quitschke, W. W. and Taheny, M. J. and Fochtmann, L. J. and Vostrov, A. A.}, citeulike-article-id = {4895977}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/10954607}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=10954607}, day = {1}, issn = {1362-4962}, journal = {Nucleic acids research}, keywords = {apbbeta, assymetry, c-terminal, ctcf, n-terminal, promoter, specificity, zinc-finger}, month = {September}, number = {17}, pages = {3370--3378}, posted-at = {2009-06-18 19:59:59}, priority = {0}, title = {Differential effect of zinc finger deletions on the binding of CTCF to the promoter of the amyloid precursor protein gene.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10954607}, volume = {28}, year = {2000} }

TY - JOUR ID - citeulike:4895977 L3 - citeulike-article-id:4895977 N2 - High levels of transcription from the amyloid precursor protein promoter are dependent on the binding of CTCF to the APBbeta core recognition sequence located between positions -82 and -93 upstream from the transcriptional start site. CTCF comprises 727 amino acids and contains 11 zinc finger motifs arranged in tandem that are flanked by 267 amino acids on the N-terminal side and 150 amino acids on the C-terminal side. Deletion of either the N- or the C-terminal regions outside of the zinc finger domain had no detrimental effect on the binding of CTCF to APBbeta. However, internal deletions of zinc fingers 5-7 completely abolished binding. The binding of full-length CTCF generated a DNase I protected domain extending from position -78 to -116, which was interrupted by a hypersensitive site at position -99. Selective deletions from the N- and C-terminal sides of the zinc finger domain showed that the N-terminal end of the zinc finger domain was aligned toward the transcriptional start site. Furthermore, deletions of zinc fingers peripheral to the essential zinc fingers 5-7 decreased the stability of the binding complex by interrupting sequence-specific interactions. IS - 17 JF - Nucleic acids research SN - 1362-4962 EP - 3378 TI - Differential effect of zinc finger deletions on the binding of CTCF to the promoter of the amyloid precursor protein gene. VL - 28 SP - 3370 KW - apbbeta KW - assymetry KW - c-terminal KW - ctcf KW - n-terminal KW - promoter KW - specificity KW - zinc-finger AU - Quitschke, WW AU - Taheny, MJ AU - Fochtmann, LJ AU - Vostrov, AA PY - 2000/09/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/10954607 ER -