Genome-wide allele- and strand-specific expression profiling. Export

@article{citeulike:5035072, abstract = {Recent reports have shown that most of the genome is transcribed and that transcription frequently occurs concurrently on both DNA strands. In diploid genomes, the expression level of each allele conditions the degree to which sequence polymorphisms affect the phenotype. It is thus essential to quantify expression in an allele- and strand-specific manner. Using a custom-designed tiling array and a new computational approach, we piloted measuring allele- and strand-specific expression in yeast. Confident quantitative estimates of allele-specific expression were obtained for about half of the coding and non-coding transcripts of a heterozygous yeast strain, of which 371 transcripts (13\%) showed significant allelic differential expression greater than 1.5-fold. The data revealed complex allelic differential expression on opposite strands. Furthermore, combining allele-specific expression with linkage mapping enabled identifying allelic variants that act in cis and in trans to regulate allelic expression in the heterozygous strain. Our results provide the first high-resolution analysis of differential expression on all four strands of an eukaryotic genome.}, author = {Gagneur, Julien and Sinha, Himanshu and Perocchi, Fabiana and Bourgon, Richard and Huber, Wolfgang and Steinmetz, Lars M.}, citeulike-article-id = {5035072}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/msb.2009.31}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/msb200931}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19536197}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19536197}, day = {16}, doi = {10.1038/msb.2009.31}, issn = {1744-4292}, journal = {Molecular systems biology}, keywords = {allele-specific, allele-specific-expression, allelic, expression, sequencing}, month = {June}, posted-at = {2009-09-24 23:48:56}, priority = {2}, publisher = {EMBO and Nature Publishing Group}, title = {Genome-wide allele- and strand-specific expression profiling.}, url = {http://dx.doi.org/10.1038/msb.2009.31}, volume = {5}, year = {2009} }

TY - JOUR ID - citeulike:5035072 L3 - citeulike-article-id:5035072 N2 - Recent reports have shown that most of the genome is transcribed and that transcription frequently occurs concurrently on both DNA strands. In diploid genomes, the expression level of each allele conditions the degree to which sequence polymorphisms affect the phenotype. It is thus essential to quantify expression in an allele- and strand-specific manner. Using a custom-designed tiling array and a new computational approach, we piloted measuring allele- and strand-specific expression in yeast. Confident quantitative estimates of allele-specific expression were obtained for about half of the coding and non-coding transcripts of a heterozygous yeast strain, of which 371 transcripts (13%) showed significant allelic differential expression greater than 1.5-fold. The data revealed complex allelic differential expression on opposite strands. Furthermore, combining allele-specific expression with linkage mapping enabled identifying allelic variants that act in cis and in trans to regulate allelic expression in the heterozygous strain. Our results provide the first high-resolution analysis of differential expression on all four strands of an eukaryotic genome. JF - Molecular systems biology SN - 1744-4292 TI - Genome-wide allele- and strand-specific expression profiling. PB - EMBO and Nature Publishing Group VL - 5 KW - allele-specific KW - allele-specific-expression KW - allelic KW - expression KW - sequencing AU - Gagneur, Julien AU - Sinha, Himanshu AU - Perocchi, Fabiana AU - Bourgon, Richard AU - Huber, Wolfgang AU - Steinmetz, Lars PY - 2009/06/16/ UR - http://dx.doi.org/10.1038/msb.2009.31 ER -