Nucleosome positioning as a determinant of exon recognition Export

@article{citeulike:5689087, abstract = {Chromatin structure influences transcription, but its role in subsequent RNA processing is unclear. Here we present analyses of high-throughput data that imply a relationship between nucleosome positioning and exon definition. First, we have found stable nucleosome occupancy within human and Caenorhabditis elegans exons that is stronger in exons with weak splice sites. Conversely, we have found that pseudoexons—intronic sequences that are not included in mRNAs but are flanked by strong splice sites—show nucleosome depletion. Second, the ratio between nucleosome occupancy within and upstream from the exons correlates with exon-inclusion levels. Third, nucleosomes are positioned central to exons rather than proximal to splice sites. These exonic nucleosomal patterns are also observed in non-expressed genes, suggesting that nucleosome marking of exons exists in the absence of transcription. Our analysis provides a framework that contributes to the understanding of splicing on the basis of chromatin architecture.}, author = {Tilgner, Hagen and Nikolaou, Christoforos and Althammer, Sonja and Sammeth, Michael and Beato, Miguel and Valcarcel, Juan and Guigo, Roderic}, citeulike-article-id = {5689087}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nsmb.1658}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nsmb.1658}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19684599}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19684599}, day = {16}, doi = {10.1038/nsmb.1658}, issn = {1545-9993}, journal = {Nature Structural \& Molecular Biology}, keywords = {exon, intron, nucleosome, nucleosome-positioning, splicing}, month = {August}, number = {9}, pages = {996--1001}, posted-at = {2009-10-29 21:43:00}, priority = {3}, publisher = {Nature Publishing Group}, title = {Nucleosome positioning as a determinant of exon recognition}, url = {http://dx.doi.org/10.1038/nsmb.1658}, volume = {16}, year = {2009} }

TY - JOUR ID - citeulike:5689087 L3 - citeulike-article-id:5689087 N2 - Chromatin structure influences transcription, but its role in subsequent RNA processing is unclear. Here we present analyses of high-throughput data that imply a relationship between nucleosome positioning and exon definition. First, we have found stable nucleosome occupancy within human and Caenorhabditis elegans exons that is stronger in exons with weak splice sites. Conversely, we have found that pseudoexons—intronic sequences that are not included in mRNAs but are flanked by strong splice sites—show nucleosome depletion. Second, the ratio between nucleosome occupancy within and upstream from the exons correlates with exon-inclusion levels. Third, nucleosomes are positioned central to exons rather than proximal to splice sites. These exonic nucleosomal patterns are also observed in non-expressed genes, suggesting that nucleosome marking of exons exists in the absence of transcription. Our analysis provides a framework that contributes to the understanding of splicing on the basis of chromatin architecture. IS - 9 JF - Nature Structural & Molecular Biology SN - 1545-9993 EP - 1001 TI - Nucleosome positioning as a determinant of exon recognition PB - Nature Publishing Group VL - 16 SP - 996 KW - exon KW - intron KW - nucleosome KW - nucleosome-positioning KW - splicing AU - Tilgner, Hagen AU - Nikolaou, Christoforos AU - Althammer, Sonja AU - Sammeth, Michael AU - Beato, Miguel AU - Valcarcel, Juan AU - Guigo, Roderic PY - 2009/08/16/ UR - http://dx.doi.org/10.1038/nsmb.1658 ER -