Recombinational repair within heterochromatin requires ATP-dependent chromatin remodeling. Export

@article{citeulike:5821453, abstract = {Heterochromatin plays a key role in protection of chromosome integrity by suppressing homologous recombination. In Saccharomyces cerevisiae, Sir2p, Sir3p, and Sir4p are structural components of heterochromatin found at telomeres and the silent mating-type loci. Here we have investigated whether incorporation of Sir proteins into minichromosomes regulates early steps of recombinational repair in vitro. We find that addition of Sir3p to a nucleosomal substrate is sufficient to eliminate yRad51p-catalyzed formation of joints, and that this repression is enhanced by Sir2p/Sir4p. Importantly, Sir-mediated repression requires histone residues that are critical for silencing in vivo. Moreover, we demonstrate that the SWI/SNF chromatin-remodeling enzyme facilitates joint formation by evicting Sir3p, thereby promoting subsequent Rad54p-dependent formation of a strand invasion product. These results suggest that recombinational repair in the context of heterochromatin presents additional constraints that can be overcome by ATP-dependent chromatin-remodeling enzymes.}, author = {Sinha, Manisha and Watanabe, Shinya and Johnson, Aaron and Moazed, Danesh and Peterson, Craig L.}, citeulike-article-id = {5821453}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.cell.2009.07.013}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0092867409008526}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19766565}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19766565}, day = {18}, doi = {10.1016/j.cell.2009.07.013}, issn = {1097-4172}, journal = {Cell}, keywords = {chromatin, histone, rad54p, remodeling, repair, sir2p, sir3p, sir4p, swisnf}, month = {September}, number = {6}, pages = {1109--1121}, posted-at = {2009-10-26 20:29:16}, priority = {0}, title = {Recombinational repair within heterochromatin requires ATP-dependent chromatin remodeling.}, url = {http://dx.doi.org/10.1016/j.cell.2009.07.013}, volume = {138}, year = {2009} }

TY - JOUR ID - citeulike:5821453 L3 - citeulike-article-id:5821453 N2 - Heterochromatin plays a key role in protection of chromosome integrity by suppressing homologous recombination. In Saccharomyces cerevisiae, Sir2p, Sir3p, and Sir4p are structural components of heterochromatin found at telomeres and the silent mating-type loci. Here we have investigated whether incorporation of Sir proteins into minichromosomes regulates early steps of recombinational repair in vitro. We find that addition of Sir3p to a nucleosomal substrate is sufficient to eliminate yRad51p-catalyzed formation of joints, and that this repression is enhanced by Sir2p/Sir4p. Importantly, Sir-mediated repression requires histone residues that are critical for silencing in vivo. Moreover, we demonstrate that the SWI/SNF chromatin-remodeling enzyme facilitates joint formation by evicting Sir3p, thereby promoting subsequent Rad54p-dependent formation of a strand invasion product. These results suggest that recombinational repair in the context of heterochromatin presents additional constraints that can be overcome by ATP-dependent chromatin-remodeling enzymes. IS - 6 JF - Cell SN - 1097-4172 EP - 1121 TI - Recombinational repair within heterochromatin requires ATP-dependent chromatin remodeling. VL - 138 SP - 1109 KW - chromatin KW - histone KW - rad54p KW - remodeling KW - repair KW - sir2p KW - sir3p KW - sir4p KW - swisnf AU - Sinha, Manisha AU - Watanabe, Shinya AU - Johnson, Aaron AU - Moazed, Danesh AU - Peterson, Craig PY - 2009/09/18/ UR - http://dx.doi.org/10.1016/j.cell.2009.07.013 ER -