Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C). Export

@article{citeulike:915630, abstract = {The spatial organization of DNA in the cell nucleus is an emerging key contributor to genomic function. We developed 4C technology (chromosome conformation capture (3C)-on-chip), which allows for an unbiased genome-wide search for DNA loci that contact a given locus in the nuclear space. We demonstrate here that active and inactive genes are engaged in many long-range intrachromosomal interactions and can also form interchromosomal contacts. The active beta-globin locus in fetal liver preferentially contacts transcribed, but not necessarily tissue-specific, loci elsewhere on chromosome 7, whereas the inactive locus in fetal brain contacts different transcriptionally silent loci. A housekeeping gene in a gene-dense region on chromosome 8 forms long-range contacts predominantly with other active gene clusters, both in cis and in trans, and many of these intra- and interchromosomal interactions are conserved between the tissues analyzed. Our data demonstrate that chromosomes fold into areas of active chromatin and areas of inactive chromatin and establish 4C technology as a powerful tool to study nuclear architecture.}, author = {Simonis, Marieke and Klous, Petra and Splinter, Erik and Moshkin, Yuri and Willemsen, Rob and de Wit, Elzo and van Steensel, Bas and de Laat, Wouter}, citeulike-article-id = {915630}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ng1896}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ng1896}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17033623}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17033623}, day = {08}, doi = {10.1038/ng1896}, issn = {1061-4036}, journal = {Nature genetics}, keywords = {3c, 3d, 4c, beta-globin, ctcf, hub, loop}, month = {November}, number = {11}, pages = {1348--1354}, posted-at = {2009-07-06 21:16:19}, priority = {0}, publisher = {Nature Publishing Group}, title = {Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C).}, url = {http://dx.doi.org/10.1038/ng1896}, volume = {38}, year = {2006} }

TY - JOUR ID - citeulike:915630 L3 - citeulike-article-id:915630 N2 - The spatial organization of DNA in the cell nucleus is an emerging key contributor to genomic function. We developed 4C technology (chromosome conformation capture (3C)-on-chip), which allows for an unbiased genome-wide search for DNA loci that contact a given locus in the nuclear space. We demonstrate here that active and inactive genes are engaged in many long-range intrachromosomal interactions and can also form interchromosomal contacts. The active beta-globin locus in fetal liver preferentially contacts transcribed, but not necessarily tissue-specific, loci elsewhere on chromosome 7, whereas the inactive locus in fetal brain contacts different transcriptionally silent loci. A housekeeping gene in a gene-dense region on chromosome 8 forms long-range contacts predominantly with other active gene clusters, both in cis and in trans, and many of these intra- and interchromosomal interactions are conserved between the tissues analyzed. Our data demonstrate that chromosomes fold into areas of active chromatin and areas of inactive chromatin and establish 4C technology as a powerful tool to study nuclear architecture. IS - 11 JF - Nature genetics SN - 1061-4036 EP - 1354 TI - Nuclear organization of active and inactive chromatin domains uncovered by chromosome conformation capture-on-chip (4C). PB - Nature Publishing Group VL - 38 SP - 1348 KW - 3c KW - 3d KW - 4c KW - beta-globin KW - ctcf KW - hub KW - loop AU - Simonis, Marieke AU - Klous, Petra AU - Splinter, Erik AU - Moshkin, Yuri AU - Willemsen, Rob AU - de Wit, Elzo AU - van Steensel, Bas AU - de Laat, Wouter PY - 2006/11/08/ UR - http://dx.doi.org/10.1038/ng1896 ER -