Neutralization of HIV-1 by redirection of natural antibodies Export

@article{citeulike:3361282, abstract = {10.1073/pnas.0805777105 The great variability and high glycosylation of gp120 poses a great challenge for the design of a functional immune therapy. The binding region of the CD4 receptor to gp120, however, is well conserved and may constitute a target to limit viral entry and infectivity. Our strategy consists in using a preexisting pool of natural antibodies directed toward the gal(α1,3)gal disaccharide and to redirect it to HIV. We here show that using CD4-derived, gp120-binding, synthetic peptides chemically linked to gal(α1,3)gal can redirect these natural antibodies and improve the HIV-1 neutralizing activity of the CD4-derived peptides . Importantly, the binding of the CD4-gal(α1,3)gal peptides to HIV-1–infected cells conferred antibody-dependent cellular cytotoxicity after the addition of human sera. Thus, the temporary redirection of naturally occurring antibodies and their biological activities to a new antigen represents a completely new way of targeting a human disease.}, author = {Perdomo, M. F. and Levi, M. and Sallberg, M. and Vahlne, A.}, citeulike-article-id = {3361282}, journal = {Proc.Natl. Acad.Sci., USA}, keywords = {anti, antibodies, cd4, hiv, infection, natural, reduce}, number = {34}, pages = {12515--12520}, posted-at = {2008-10-01 04:13:14}, priority = {2}, title = {Neutralization of HIV-1 by redirection of natural antibodies}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:3361282 L3 - citeulike-article-id:3361282 N2 - 10.1073/pnas.0805777105 The great variability and high glycosylation of gp120 poses a great challenge for the design of a functional immune therapy. The binding region of the CD4 receptor to gp120, however, is well conserved and may constitute a target to limit viral entry and infectivity. Our strategy consists in using a preexisting pool of natural antibodies directed toward the gal(α1,3)gal disaccharide and to redirect it to HIV. We here show that using CD4-derived, gp120-binding, synthetic peptides chemically linked to gal(α1,3)gal can redirect these natural antibodies and improve the HIV-1 neutralizing activity of the CD4-derived peptides . Importantly, the binding of the CD4-gal(α1,3)gal peptides to HIV-1–infected cells conferred antibody-dependent cellular cytotoxicity after the addition of human sera. Thus, the temporary redirection of naturally occurring antibodies and their biological activities to a new antigen represents a completely new way of targeting a human disease. IS - 34 JF - Proc.Natl. Acad.Sci., USA EP - 12520 TI - Neutralization of HIV-1 by redirection of natural antibodies VL - 105 SP - 12515 KW - anti KW - antibodies KW - cd4 KW - hiv KW - infection KW - natural KW - reduce AU - Perdomo, MF AU - Levi, M AU - Sallberg, M AU - Vahlne, A PY - 2008/// ER -