The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics. Export

@article{citeulike:3851034, abstract = {It was recently reported that the production of Reactive Oxygen Species (ROS) is a common mechanism of cell death induced by bactericidal antibiotics. Here we show that triggering the Escherichia coli chromosomal toxin-antitoxin system mazEF is an additional determinant in the mode of action of some antibiotics. We treated E. coli cultures by antibiotics belonging to one of two groups: (i) Inhibitors of transcription and/or translation, and (ii) DNA damaging. We found that antibiotics of both groups caused: (i) mazEF-mediated cell death, and (ii) the production of ROS through MazF action. However, only antibiotics of the first group caused mazEF-mediated cell death that is ROS-dependent, whereas those of the second group caused mazEF-mediated cell death by an ROS-independent pathway. Furthermore, our results showed that the mode of action of antibiotics was determined by the ability of E. coli cells to communicate through the signaling molecule Extracellular Death Factor (EDF) participating in mazEF induction.}, author = {Kolodkin-Gal, I. and Sat, B. and Keshet, A. and Engelberg-Kulka, H.}, citeulike-article-id = {3851034}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pbio.0060319}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19090622}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19090622}, day = {16}, doi = {10.1371/journal.pbio.0060319}, issn = {1545-7885}, journal = {PLoS biology}, keywords = {antibiotics, maze, mazf, toxin-antitoxin}, month = {December}, number = {12}, posted-at = {2009-01-05 16:54:24}, priority = {2}, title = {The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics.}, url = {http://dx.doi.org/10.1371/journal.pbio.0060319}, volume = {6}, year = {2008} }

TY - JOUR ID - citeulike:3851034 L3 - citeulike-article-id:3851034 N2 - It was recently reported that the production of Reactive Oxygen Species (ROS) is a common mechanism of cell death induced by bactericidal antibiotics. Here we show that triggering the Escherichia coli chromosomal toxin-antitoxin system mazEF is an additional determinant in the mode of action of some antibiotics. We treated E. coli cultures by antibiotics belonging to one of two groups: (i) Inhibitors of transcription and/or translation, and (ii) DNA damaging. We found that antibiotics of both groups caused: (i) mazEF-mediated cell death, and (ii) the production of ROS through MazF action. However, only antibiotics of the first group caused mazEF-mediated cell death that is ROS-dependent, whereas those of the second group caused mazEF-mediated cell death by an ROS-independent pathway. Furthermore, our results showed that the mode of action of antibiotics was determined by the ability of E. coli cells to communicate through the signaling molecule Extracellular Death Factor (EDF) participating in mazEF induction. IS - 12 JF - PLoS biology SN - 1545-7885 TI - The communication factor EDF and the toxin-antitoxin module mazEF determine the mode of action of antibiotics. VL - 6 KW - antibiotics KW - maze KW - mazf KW - toxin-antitoxin AU - Kolodkin-Gal, I AU - Sat, B AU - Keshet, A AU - Engelberg-Kulka, H PY - 2008/12/16/ UR - http://dx.doi.org/10.1371/journal.pbio.0060319 ER -