Modulation of CD103 Expression on Human Colon Carcinoma-Specific CTL Export

@article{citeulike:1115042, abstract = {Recent results have shown a correlation between survival and frequency of tumor-infiltrating T cells in colorectal cancer patients. However, the mechanisms controlling the ability of human T lymphocytes to infiltrate colon carcinoma remain unclear. Although, it is known that expression of the integrin CD103{alpha}E/[beta]7 by intraepithelial lymphocytes controls the retention of lymphocytes in epithelial layers, very little is known about the expression of intestinal homing receptors in human T lymphocytes. In particular, it remains unknown whether expression of CD103/[beta]7 by human colon cancer-specific T lymphocytes is controlled by recognition of tumor Ags and is imprinted during T cell priming, facilitating its expression during memory T cell activation. In this study, we demonstrate that expression of CD103/[beta]7 in human colon carcinoma-specific CTL is synergistically enhanced by the simultaneous TGF-[beta]1 stimulation and Ag recognition. These results were confirmed by using a panel of human CTL clones. Finally, we show that priming of naive CD8+ T cells in the presence of TGF-[beta]1 ensures up-regulation of CD103/[beta]7 in recall responses, at concentrations of TGF-[beta]1 significantly lower than those required by memory T cells primed in the absence of TGF-[beta]1. These results indicate a role of TGF-[beta]1 during T cell priming in modulating expression of CD103/[beta]7 and controlling retention of human memory CD8+ T cells into tumor epithelium.}, author = {Ling, Khoon-Lin and Dulphy, Nicolas and Bahl, Pru and Salio, Mariolina and Maskell, Kevin and Piris, Juan and Warren, Bryan F. and George, Bruce D. and Mortensen, Neil J. and Cerundolo, Vincenzo}, citeulike-article-id = {1115042}, citeulike-linkout-0 = {http://www.jimmunol.org/cgi/content/abstract/178/5/2908}, day = {1}, journal = {J Immunol}, keywords = {cancer, integrin}, month = {March}, number = {5}, pages = {2908--2915}, posted-at = {2007-02-20 20:18:20}, priority = {3}, title = {Modulation of CD103 Expression on Human Colon Carcinoma-Specific CTL}, url = {http://www.jimmunol.org/cgi/content/abstract/178/5/2908}, volume = {178}, year = {2007} }

TY - JOUR ID - citeulike:1115042 L3 - citeulike-article-id:1115042 N2 - Recent results have shown a correlation between survival and frequency of tumor-infiltrating T cells in colorectal cancer patients. However, the mechanisms controlling the ability of human T lymphocytes to infiltrate colon carcinoma remain unclear. Although, it is known that expression of the integrin CD103{alpha}E/[beta]7 by intraepithelial lymphocytes controls the retention of lymphocytes in epithelial layers, very little is known about the expression of intestinal homing receptors in human T lymphocytes. In particular, it remains unknown whether expression of CD103/[beta]7 by human colon cancer-specific T lymphocytes is controlled by recognition of tumor Ags and is imprinted during T cell priming, facilitating its expression during memory T cell activation. In this study, we demonstrate that expression of CD103/[beta]7 in human colon carcinoma-specific CTL is synergistically enhanced by the simultaneous TGF-[beta]1 stimulation and Ag recognition. These results were confirmed by using a panel of human CTL clones. Finally, we show that priming of naive CD8+ T cells in the presence of TGF-[beta]1 ensures up-regulation of CD103/[beta]7 in recall responses, at concentrations of TGF-[beta]1 significantly lower than those required by memory T cells primed in the absence of TGF-[beta]1. These results indicate a role of TGF-[beta]1 during T cell priming in modulating expression of CD103/[beta]7 and controlling retention of human memory CD8+ T cells into tumor epithelium. IS - 5 JF - J Immunol EP - 2915 TI - Modulation of CD103 Expression on Human Colon Carcinoma-Specific CTL VL - 178 SP - 2908 KW - cancer KW - integrin AU - Ling, Khoon-Lin AU - Dulphy, Nicolas AU - Bahl, Pru AU - Salio, Mariolina AU - Maskell, Kevin AU - Piris, Juan AU - Warren, Bryan AU - George, Bruce AU - Mortensen, Neil AU - Cerundolo, Vincenzo PY - 2007/03/01/ UR - http://www.jimmunol.org/cgi/content/abstract/178/5/2908 ER -