CTLA-4 Differentially Regulates the Immunological Synapse in CD4 T Cell Subsets Export

@article{citeulike:1235826, abstract = {Primary murine Th1 and Th2 cells differ in the organization of the immunological synapse, with Th1 cells, but not Th2 cells, clustering signaling molecules at the T cell/B cell synapse site. We sought to determine whether differential costimulatory signals could account for the differences observed. We found that Th2 cells express higher levels of CTLA-4 than Th1 cells, and demonstrated that Th2 cells lacking CTLA-4 are now able to cluster the TCR with the same frequency as Th1 cells. Furthermore, reconstitution of CTLA-4 into CTLA-4-deficient Th2 cells, or into Th1 cells, inhibits the clustering of the TCR. We have also shown that Th2 cells, but not Th1 cells, show variations in the organization of the immunological synapse depending on levels of expression of CD80/CD86 on the APC. These studies demonstrate a unique role for CTLA-4 as a critical regulator of Th2 cells and the immunological synapse.}, author = {Jackman, Rachael P. and Balamuth, Fran and Bottomly, Kim}, citeulike-article-id = {1235826}, citeulike-linkout-0 = {http://www.jimmunol.org/cgi/content/abstract/178/9/5543}, day = {1}, journal = {J Immunol}, keywords = {ctla-4, th1th2}, month = {May}, number = {9}, pages = {5543--5551}, posted-at = {2007-04-19 01:59:50}, priority = {2}, title = {CTLA-4 Differentially Regulates the Immunological Synapse in CD4 T Cell Subsets}, url = {http://www.jimmunol.org/cgi/content/abstract/178/9/5543}, volume = {178}, year = {2007} }

TY - JOUR ID - citeulike:1235826 L3 - citeulike-article-id:1235826 N2 - Primary murine Th1 and Th2 cells differ in the organization of the immunological synapse, with Th1 cells, but not Th2 cells, clustering signaling molecules at the T cell/B cell synapse site. We sought to determine whether differential costimulatory signals could account for the differences observed. We found that Th2 cells express higher levels of CTLA-4 than Th1 cells, and demonstrated that Th2 cells lacking CTLA-4 are now able to cluster the TCR with the same frequency as Th1 cells. Furthermore, reconstitution of CTLA-4 into CTLA-4-deficient Th2 cells, or into Th1 cells, inhibits the clustering of the TCR. We have also shown that Th2 cells, but not Th1 cells, show variations in the organization of the immunological synapse depending on levels of expression of CD80/CD86 on the APC. These studies demonstrate a unique role for CTLA-4 as a critical regulator of Th2 cells and the immunological synapse. IS - 9 JF - J Immunol EP - 5551 TI - CTLA-4 Differentially Regulates the Immunological Synapse in CD4 T Cell Subsets VL - 178 SP - 5543 KW - ctla-4 KW - th1th2 AU - Jackman, Rachael AU - Balamuth, Fran AU - Bottomly, Kim PY - 2007/05/01/ UR - http://www.jimmunol.org/cgi/content/abstract/178/9/5543 ER -