PKCtheta cooperates with atypical PKCzeta and PKCiota in NF-kappaB transactivation of T lymphocytes. Export

@article{citeulike:1429559, abstract = {Using yeast two-hybrid, we isolated atypical PKCzeta as a PKCtheta-interacting kinase and demonstrated that it selectively interacted with, and was phosphorylated by, PKCtheta. Importantly, however, both atypical PKCzeta and PKCiota were functionally required in TCR/CD28-mediated activation of NF-kappaB downstream of PKCtheta in Jurkat T cells albeit, activation responses of PKCzeta-deficient CD3(+) T cells were comparable with wildtype controls. This normal activation thresholds of PKCzeta(-/-) T cells suggested that PKCiota, the closest structural relative, might play a compensatory role in TCR/CD28-induced signalling. Consistently, both PKCzeta and PKCiota resided in the plasma membrane lipid raft microdomains of Jurkat as well as primary mouse CD3(+) T cells. Thus, PKCtheta, the established constituent of the immunological synapse, physically and functionally interacted with PKCzeta and PKCiota. Together, these data demonstrate that atypical PKCzeta/iota isotypes serve as direct downstream targets of PKCtheta in the signalling pathway leading to NF-kappaB activation in T lymphocytes.}, address = {Department for Medical Genetics, Molecular and Clinical Pharmacology, Innsbruck Medical University, Schoepfstra{\ss}e 41, A-6020 Innsbruck, Austria.}, author = {Gruber, Thomas and Fresser, Friedrich and Jenny, Marcel and Uberall, Florian and Leitges, Michael and Baier, Gottfried}, citeulike-article-id = {1429559}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.molimm.2007.05.003}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17588663}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17588663}, day = {21}, doi = {10.1016/j.molimm.2007.05.003}, issn = {0161-5890}, journal = {Mol Immunol}, keywords = {pkc, pkctheta}, month = {June}, posted-at = {2007-07-02 22:51:57}, priority = {2}, title = {PKCtheta cooperates with atypical PKCzeta and PKCiota in NF-kappaB transactivation of T lymphocytes.}, url = {http://dx.doi.org/10.1016/j.molimm.2007.05.003}, year = {2007} }

TY - JOUR ID - citeulike:1429559 L3 - citeulike-article-id:1429559 N2 - Using yeast two-hybrid, we isolated atypical PKCzeta as a PKCtheta-interacting kinase and demonstrated that it selectively interacted with, and was phosphorylated by, PKCtheta. Importantly, however, both atypical PKCzeta and PKCiota were functionally required in TCR/CD28-mediated activation of NF-kappaB downstream of PKCtheta in Jurkat T cells albeit, activation responses of PKCzeta-deficient CD3(+) T cells were comparable with wildtype controls. This normal activation thresholds of PKCzeta(-/-) T cells suggested that PKCiota, the closest structural relative, might play a compensatory role in TCR/CD28-induced signalling. Consistently, both PKCzeta and PKCiota resided in the plasma membrane lipid raft microdomains of Jurkat as well as primary mouse CD3(+) T cells. Thus, PKCtheta, the established constituent of the immunological synapse, physically and functionally interacted with PKCzeta and PKCiota. Together, these data demonstrate that atypical PKCzeta/iota isotypes serve as direct downstream targets of PKCtheta in the signalling pathway leading to NF-kappaB activation in T lymphocytes. JF - Mol Immunol SN - 0161-5890 AD - Department for Medical Genetics, Molecular and Clinical Pharmacology, Innsbruck Medical University, Schoepfstraße 41, A-6020 Innsbruck, Austria. TI - PKCtheta cooperates with atypical PKCzeta and PKCiota in NF-kappaB transactivation of T lymphocytes. KW - pkc KW - pkctheta AU - Gruber, Thomas AU - Fresser, Friedrich AU - Jenny, Marcel AU - Uberall, Florian AU - Leitges, Michael AU - Baier, Gottfried PY - 2007/06/21/ UR - http://dx.doi.org/10.1016/j.molimm.2007.05.003 ER -