Immune synapse formation requires ZAP-70 recruitment by ezrin and CD43 removal by moesin Export

@article{citeulike:1943211, abstract = {Immunological synapse (IS) formation involves receptorligand pair clustering and intracellular signaling molecule recruitment with a coincident removal of other membrane proteins away from the IS. As microfilamentmembrane linkage is critical to this process, we investigated the involvement of ezrin and moesin, the two ezrin/radixin/moesin proteins expressed in T cells. We demonstrate that ezrin and moesin, which are generally believed to be functionally redundant, are differentially localized and have important and complementary functions in IS formation. Specifically, we find that ezrin directly interacts with and recruits the signaling kinase ZAP-70 to the IS. Furthermore, the activation of ezrin by phosphorylation is essential for this process. In contrast, moesin dephosphorylation and removal, along with CD43, are necessary to prepare a region of the cell cortex for IS. Thus, ezrin and moesin have distinct and critical functions in the T cell cortex during IS formation. 10.1083/jcb.200707199}, author = {Ilani, Tal and Khanna, Chand and Zhou, Ming and Veenstra, Timothy D. and Bretscher, Anthony}, citeulike-article-id = {1943211}, citeulike-linkout-0 = {http://dx.doi.org/10.1083/jcb.200707199}, citeulike-linkout-1 = {http://www.jcb.org/cgi/content/abstract/179/4/733}, day = {19}, doi = {10.1083/jcb.200707199}, journal = {J. Cell Biol.}, keywords = {cd43, ezrin, moesin, synapse, zap-70}, month = {November}, number = {4}, pages = {733--746}, posted-at = {2007-11-20 12:39:55}, priority = {2}, title = {Immune synapse formation requires ZAP-70 recruitment by ezrin and CD43 removal by moesin}, url = {http://dx.doi.org/10.1083/jcb.200707199}, volume = {179}, year = {2007} }

TY - JOUR ID - citeulike:1943211 L3 - citeulike-article-id:1943211 N2 - Immunological synapse (IS) formation involves receptorligand pair clustering and intracellular signaling molecule recruitment with a coincident removal of other membrane proteins away from the IS. As microfilamentmembrane linkage is critical to this process, we investigated the involvement of ezrin and moesin, the two ezrin/radixin/moesin proteins expressed in T cells. We demonstrate that ezrin and moesin, which are generally believed to be functionally redundant, are differentially localized and have important and complementary functions in IS formation. Specifically, we find that ezrin directly interacts with and recruits the signaling kinase ZAP-70 to the IS. Furthermore, the activation of ezrin by phosphorylation is essential for this process. In contrast, moesin dephosphorylation and removal, along with CD43, are necessary to prepare a region of the cell cortex for IS. Thus, ezrin and moesin have distinct and critical functions in the T cell cortex during IS formation. 10.1083/jcb.200707199 IS - 4 JF - J. Cell Biol. EP - 746 TI - Immune synapse formation requires ZAP-70 recruitment by ezrin and CD43 removal by moesin VL - 179 SP - 733 KW - cd43 KW - ezrin KW - moesin KW - synapse KW - zap-70 AU - Ilani, Tal AU - Khanna, Chand AU - Zhou, Ming AU - Veenstra, Timothy AU - Bretscher, Anthony PY - 2007/11/19/ UR - http://dx.doi.org/10.1083/jcb.200707199 ER -