Stromal mesenteric lymph node cells are essential for the generation of gut-homing T cells in vivo Export

@article{citeulike:3417284, abstract = {T cells primed in the gut-draining mesenteric lymph nodes (mLN) are imprinted to express {alpha}4{beta}7-integrin and chemokine receptor CCR9, thereby enabling lymphocytes to migrate to the small intestine. In vitro activation by intestinal dendritic cells (DC) or addition of retinoic acid (RA) is sufficient to instruct expression of these gut-homing molecules. We report that in vivo stroma cells, but not DC, allow the mLN to induce the generation of gut tropism. Peripheral LN (pLN) transplanted into the gut mesenteries fail to support the generation of gut-homing T cells, even though gut-derived DC enter the transplants and prime T cells. DC that fail to induce {alpha}4{beta}7-integrin and CCR9 in vitro readily induce these factors in vivo upon injection into mLN afferent lymphatics. Moreover, uniquely mesenteric but not pLN stroma cells express high levels of RA-producing enzymes and support induction of CCR9 on activated T cells in vitro. These results demonstrate a hitherto unrecognized contribution of stromal cell delivered signals, including RA, on the imprinting of tissue tropism in vivo. 10.1084/jem.20080039}, author = {Hammerschmidt, Swantje I. and Ahrendt, Manuela and Bode, Ulrike and Wahl, Benjamin and Kremmer, Elisabeth and Forster, Reinhold and Pabst, Oliver}, citeulike-article-id = {3417284}, citeulike-linkout-0 = {http://dx.doi.org/10.1084/jem.20080039}, citeulike-linkout-1 = {http://jem.rupress.org/content/jem.20080039v1/.abstract}, citeulike-linkout-2 = {http://jem.rupress.org/content/jem.20080039v1/.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/18852290}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=18852290}, day = {13}, doi = {10.1084/jem.20080039}, issn = {1540-9538}, journal = {J. Exp. Med.}, keywords = {a4b7, gut}, month = {October}, pages = {jem.20080039+}, posted-at = {2008-10-16 12:17:14}, priority = {2}, title = {Stromal mesenteric lymph node cells are essential for the generation of gut-homing T cells in vivo}, url = {http://dx.doi.org/10.1084/jem.20080039}, year = {2008} }

TY - JOUR ID - citeulike:3417284 L3 - citeulike-article-id:3417284 N2 - T cells primed in the gut-draining mesenteric lymph nodes (mLN) are imprinted to express {alpha}4{beta}7-integrin and chemokine receptor CCR9, thereby enabling lymphocytes to migrate to the small intestine. In vitro activation by intestinal dendritic cells (DC) or addition of retinoic acid (RA) is sufficient to instruct expression of these gut-homing molecules. We report that in vivo stroma cells, but not DC, allow the mLN to induce the generation of gut tropism. Peripheral LN (pLN) transplanted into the gut mesenteries fail to support the generation of gut-homing T cells, even though gut-derived DC enter the transplants and prime T cells. DC that fail to induce {alpha}4{beta}7-integrin and CCR9 in vitro readily induce these factors in vivo upon injection into mLN afferent lymphatics. Moreover, uniquely mesenteric but not pLN stroma cells express high levels of RA-producing enzymes and support induction of CCR9 on activated T cells in vitro. These results demonstrate a hitherto unrecognized contribution of stromal cell delivered signals, including RA, on the imprinting of tissue tropism in vivo. 10.1084/jem.20080039 JF - J. Exp. Med. SN - 1540-9538 TI - Stromal mesenteric lymph node cells are essential for the generation of gut-homing T cells in vivo SP - jem.20080039 KW - a4b7 KW - gut AU - Hammerschmidt, Swantje AU - Ahrendt, Manuela AU - Bode, Ulrike AU - Wahl, Benjamin AU - Kremmer, Elisabeth AU - Forster, Reinhold AU - Pabst, Oliver PY - 2008/10/13/ UR - http://dx.doi.org/10.1084/jem.20080039 ER -