Regulation of conformer-specific activation of the integrin LFA-1 by a chemokine-triggered Rho signaling module. Export

@article{citeulike:3883742, abstract = {Regulation of the affinity of the beta(2) integrin LFA-1 by chemokines is critical to lymphocyte trafficking, but the signaling mechanisms that control this process are not well understood. Here we investigated the signaling events controlling LFA-1 affinity triggering by chemokines in human primary T lymphocytes. We found that the small GTPase Rac1 mediated chemokine-induced LFA-1 affinity triggering and lymphocyte arrest in high endothelial venules. Unexpectedly, another Rho family member, Cdc42, negatively regulated LFA-1 activation. The Rho effectors PLD1 and PIP5KC were also critical to LFA-1 affinity modulation. Notably, PIP5KC was found to specifically control the transition of LFA-1 from an extended low-intermediate state to a high-affinity state, which correlated with lymphocyte arrest. Thus, chemokines control lymphocyte trafficking by triggering a Rho-dependent signaling cascade leading to conformer-specific modulation of LFA-1 affinity.}, author = {Bolomini-Vittori, Matteo and Montresor, Alessio and Giagulli, Cinzia and Staunton, Donald and Rossi, Barbara and Martinello, Marianna and Constantin, Gabriela and Laudanna, Carlo}, citeulike-article-id = {3883742}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/ni.1691}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/ni.1691}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19136961}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19136961}, day = {11}, doi = {10.1038/ni.1691}, issn = {1529-2916}, journal = {Nature immunology}, keywords = {chemokine, lfa-1, rac}, month = {January}, posted-at = {2009-01-13 21:10:21}, priority = {2}, title = {Regulation of conformer-specific activation of the integrin LFA-1 by a chemokine-triggered Rho signaling module.}, url = {http://dx.doi.org/10.1038/ni.1691}, year = {2009} }

TY - JOUR ID - citeulike:3883742 L3 - citeulike-article-id:3883742 N2 - Regulation of the affinity of the beta(2) integrin LFA-1 by chemokines is critical to lymphocyte trafficking, but the signaling mechanisms that control this process are not well understood. Here we investigated the signaling events controlling LFA-1 affinity triggering by chemokines in human primary T lymphocytes. We found that the small GTPase Rac1 mediated chemokine-induced LFA-1 affinity triggering and lymphocyte arrest in high endothelial venules. Unexpectedly, another Rho family member, Cdc42, negatively regulated LFA-1 activation. The Rho effectors PLD1 and PIP5KC were also critical to LFA-1 affinity modulation. Notably, PIP5KC was found to specifically control the transition of LFA-1 from an extended low-intermediate state to a high-affinity state, which correlated with lymphocyte arrest. Thus, chemokines control lymphocyte trafficking by triggering a Rho-dependent signaling cascade leading to conformer-specific modulation of LFA-1 affinity. JF - Nature immunology SN - 1529-2916 TI - Regulation of conformer-specific activation of the integrin LFA-1 by a chemokine-triggered Rho signaling module. KW - chemokine KW - lfa-1 KW - rac AU - Bolomini-Vittori, Matteo AU - Montresor, Alessio AU - Giagulli, Cinzia AU - Staunton, Donald AU - Rossi, Barbara AU - Martinello, Marianna AU - Constantin, Gabriela AU - Laudanna, Carlo PY - 2009/01/11/ UR - http://dx.doi.org/10.1038/ni.1691 ER -