Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells. Export

@article{citeulike:4285240, abstract = {Adhesion and motility of mammalian leukocytes are essential requirements for innate and adaptive immune defence mechanisms. We show here that the guanine nucleotide exchange factor cytohesin-1 which had previously been demonstrated to be an important component of beta-2 integrin activation in lymphocytes, regulates the activation of the small GTPase RhoA in primary dendritic cells (DC). Cytohesin-1 and RhoA are both required for the induction of chemokine-dependent conformational changes of the integrin beta-2 subunit of DC during adhesion under physiological flow conditions. Furthermore, employment of RNAi in murine BM-DC revealed that interference with cytohesin-1 signaling impairs migration of wild type dendritic cells in complex 3D environments and in vivo. This phentoype was not observed in the complete absence of integrins. We thus demonstrate an essential role of cytohesin-1/RhoA during amoeboid migration in the presence of integrins and further suggest that DCs without integrins switch to a different migration mode.}, author = {Quast, Thomas and Tappertzhofen, Barbara and Schild, Cora and Grell, Jessica and Czeloth, Niklas and Forster, Reinhold and Alon, Ronen and Fraemohs, Line and Dreck, Katrin and Weber, Christian and Lammermann, Tim and Sixt, Michael and Kolanus, Waldemar}, citeulike-article-id = {4285240}, citeulike-linkout-0 = {http://dx.doi.org/10.1182/blood-2008-08-176123}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19346499}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19346499}, day = {3}, doi = {10.1182/blood-2008-08-176123}, issn = {1528-0020}, journal = {Blood}, keywords = {dc, integrin, migration}, month = {April}, posted-at = {2009-04-07 21:39:44}, priority = {2}, title = {Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells.}, url = {http://dx.doi.org/10.1182/blood-2008-08-176123}, year = {2009} }

TY - JOUR ID - citeulike:4285240 L3 - citeulike-article-id:4285240 N2 - Adhesion and motility of mammalian leukocytes are essential requirements for innate and adaptive immune defence mechanisms. We show here that the guanine nucleotide exchange factor cytohesin-1 which had previously been demonstrated to be an important component of beta-2 integrin activation in lymphocytes, regulates the activation of the small GTPase RhoA in primary dendritic cells (DC). Cytohesin-1 and RhoA are both required for the induction of chemokine-dependent conformational changes of the integrin beta-2 subunit of DC during adhesion under physiological flow conditions. Furthermore, employment of RNAi in murine BM-DC revealed that interference with cytohesin-1 signaling impairs migration of wild type dendritic cells in complex 3D environments and in vivo. This phentoype was not observed in the complete absence of integrins. We thus demonstrate an essential role of cytohesin-1/RhoA during amoeboid migration in the presence of integrins and further suggest that DCs without integrins switch to a different migration mode. JF - Blood SN - 1528-0020 TI - Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells. KW - dc KW - integrin KW - migration AU - Quast, Thomas AU - Tappertzhofen, Barbara AU - Schild, Cora AU - Grell, Jessica AU - Czeloth, Niklas AU - Forster, Reinhold AU - Alon, Ronen AU - Fraemohs, Line AU - Dreck, Katrin AU - Weber, Christian AU - Lammermann, Tim AU - Sixt, Michael AU - Kolanus, Waldemar PY - 2009/04/03/ UR - http://dx.doi.org/10.1182/blood-2008-08-176123 ER -