Dynamic Changes During the Immune Response in T Cell-Antigen-presenting Cell Clusters Isolated from Lymph Nodes Export

@article{citeulike:6029126, abstract = {Activation of antigen-specific T cells by mature dendritic cells in secondary lymphoid organs is a key control point of the adaptive immune response. Here we describe the ex vivo isolation of preformed multicellular clusters between T cells and antigen-presenting cells. Adoptively transferred, antigen-specific T cells segregated into individual clusters where their activation and proliferation was initiated in vivo. Transit of the T cell cohort through the cluster compartment required 32-36 h. The precise timing of the response to agonistic epitopes was remarkably invariant regardless of the T cell lineage, the major histocompatibility complex haplotype, and the antigen dose. Interestingly, initiation of cell division of T cells specific for a subdominant epitope and a weak agonist was delayed by 6 h. The results provide a basis for the analysis of short range, mutual cell-cell interactions within such confined microenvironments. 10.1084/jem.20021512}, author = {Hommel, Mirja and Kyewski, Bruno}, citeulike-article-id = {6029126}, citeulike-linkout-0 = {http://dx.doi.org/10.1084/jem.20021512}, citeulike-linkout-1 = {http://jem.rupress.org/content/197/3/269.abstract}, citeulike-linkout-2 = {http://jem.rupress.org/content/197/3/269.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/12566411}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=12566411}, day = {3}, doi = {10.1084/jem.20021512}, journal = {J. Exp. Med.}, keywords = {icam, lymph\_node}, month = {February}, number = {3}, pages = {269--280}, posted-at = {2009-10-28 21:57:05}, priority = {2}, title = {Dynamic Changes During the Immune Response in T Cell-Antigen-presenting Cell Clusters Isolated from Lymph Nodes}, url = {http://dx.doi.org/10.1084/jem.20021512}, volume = {197}, year = {2003} }

TY - JOUR ID - citeulike:6029126 L3 - citeulike-article-id:6029126 N2 - Activation of antigen-specific T cells by mature dendritic cells in secondary lymphoid organs is a key control point of the adaptive immune response. Here we describe the ex vivo isolation of preformed multicellular clusters between T cells and antigen-presenting cells. Adoptively transferred, antigen-specific T cells segregated into individual clusters where their activation and proliferation was initiated in vivo. Transit of the T cell cohort through the cluster compartment required 32-36 h. The precise timing of the response to agonistic epitopes was remarkably invariant regardless of the T cell lineage, the major histocompatibility complex haplotype, and the antigen dose. Interestingly, initiation of cell division of T cells specific for a subdominant epitope and a weak agonist was delayed by 6 h. The results provide a basis for the analysis of short range, mutual cell-cell interactions within such confined microenvironments. 10.1084/jem.20021512 IS - 3 JF - J. Exp. Med. EP - 280 TI - Dynamic Changes During the Immune Response in T Cell-Antigen-presenting Cell Clusters Isolated from Lymph Nodes VL - 197 SP - 269 KW - icam KW - lymph_node AU - Hommel, Mirja AU - Kyewski, Bruno PY - 2003/02/03/ UR - http://dx.doi.org/10.1084/jem.20021512 ER -