MTOC translocation modulates IS formation and controls sustained T cell signaling. Export

@article{citeulike:6056702, abstract = {The translocation of the microtubule-organizing center (MTOC) toward the nascent immune synapse (IS) is an early step in lymphocyte activation initiated by T cell receptor (TCR) signaling. The molecular mechanisms that control the physical movement of the lymphocyte MTOC remain largely unknown. We have studied the role of the dynein-dynactin complex, a microtubule-based molecular motor, in the process of T cell activation during T cell antigen-presenting cell cognate immune interactions. Impairment of dynein-dynactin complex activity, either by overexpressing the p50-dynamitin component of dynactin to disrupt the complex or by knocking down dynein heavy chain expression to prevent its formation, inhibited MTOC translocation after TCR antigen priming. This resulted in a strong reduction in the phosphorylation of molecules such as zeta chain-associated protein kinase 70 (ZAP70), linker of activated T cells (LAT), and Vav1; prevented the supply of molecules to the IS from intracellular pools, resulting in a disorganized and dysfunctional IS architecture; and impaired interleukin-2 production. Together, these data reveal MTOC translocation as an important mechanism underlying IS formation and sustained T cell signaling.}, author = {Mart\'{\i}n-C\'{o}freces, Noa B. and Robles-Valero, Javier and Cabrero, J. Rom\'{a}n and Mittelbrunn, Mar\'{\i}a and Gord\'{o}n-Alonso, M\'{o}nica and Sung, Ching-Hwa H. and Alarc\'{o}n, Balbino and V\'{a}zquez, Jes\'{u}s and S\'{a}nchez-Madrid, Francisco}, citeulike-article-id = {6056702}, citeulike-linkout-0 = {http://dx.doi.org/10.1083/jcb.200801014}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18779373}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18779373}, day = {8}, doi = {10.1083/jcb.200801014}, issn = {1540-8140}, journal = {The Journal of cell biology}, keywords = {dynein, mtoc, synapse}, month = {September}, number = {5}, pages = {951--962}, posted-at = {2009-11-02 15:48:54}, priority = {2}, title = {MTOC translocation modulates IS formation and controls sustained T cell signaling.}, url = {http://dx.doi.org/10.1083/jcb.200801014}, volume = {182}, year = {2008} }

TY - JOUR ID - citeulike:6056702 L3 - citeulike-article-id:6056702 N2 - The translocation of the microtubule-organizing center (MTOC) toward the nascent immune synapse (IS) is an early step in lymphocyte activation initiated by T cell receptor (TCR) signaling. The molecular mechanisms that control the physical movement of the lymphocyte MTOC remain largely unknown. We have studied the role of the dynein-dynactin complex, a microtubule-based molecular motor, in the process of T cell activation during T cell antigen-presenting cell cognate immune interactions. Impairment of dynein-dynactin complex activity, either by overexpressing the p50-dynamitin component of dynactin to disrupt the complex or by knocking down dynein heavy chain expression to prevent its formation, inhibited MTOC translocation after TCR antigen priming. This resulted in a strong reduction in the phosphorylation of molecules such as zeta chain-associated protein kinase 70 (ZAP70), linker of activated T cells (LAT), and Vav1; prevented the supply of molecules to the IS from intracellular pools, resulting in a disorganized and dysfunctional IS architecture; and impaired interleukin-2 production. Together, these data reveal MTOC translocation as an important mechanism underlying IS formation and sustained T cell signaling. IS - 5 JF - The Journal of cell biology SN - 1540-8140 EP - 962 TI - MTOC translocation modulates IS formation and controls sustained T cell signaling. VL - 182 SP - 951 KW - dynein KW - mtoc KW - synapse AU - Martín-Cófreces, Noa AU - Robles-Valero, Javier AU - Cabrero, Román AU - Mittelbrunn, María AU - Gordón-Alonso, Mónica AU - Sung, Ching-Hwa AU - Alarcón, Balbino AU - Vázquez, Jesús AU - Sánchez-Madrid, Francisco PY - 2008/09/08/ UR - http://dx.doi.org/10.1083/jcb.200801014 ER -